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To enhance ionization efficiency, pre\u2010column derivatization was employed using sulfhydryl nucleophiles (\u03b2\u2010mercaptoethanol and 3\u2010mercaptopropionic acid) via Michael addition. Derivatization with mercaptopropionic acid significantly improved mass spectrometric response (50\u2010fold increase) and minimized matrix effects compared to mercaptoethanol. The method, validated per FDA guidelines, exhibited excellent linearity (\n                    <jats:italic>r<\/jats:italic>\n                    \u00a0\u2265\u00a00.995) from 3 to 150\u00a0ng\/mL, with accuracy within \u221210.6% to 4.3% and precision between 1.29% and 5.34%. The lower limit of quantification was 3\u00a0ng\/mL. Extraction recovery and matrix effects ranged from 99.84% to 102.06% and 92.75% to 98.98%, respectively, with derivatives demonstrating good stability. Cellular uptake studies in MCF\u20107 cells revealed time\u2010dependent but limited intracellular accumulation of juglone, with the majority retained in the culture medium. This robust derivatization\u2010based UHPLC\u2013MS\/MS approach provides a reliable tool for investigating juglone's pharmacokinetics and toxicological mechanisms.\n                  <\/jats:p>","DOI":"10.1002\/jssc.70409","type":"journal-article","created":{"date-parts":[[2026,4,15]],"date-time":"2026-04-15T05:21:51Z","timestamp":1776230511000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["A Sensitive and Selective UHPLC\u2013MS\/MS Method Based on Mercaptopropionic Acid Derivatization for Quantification of Juglone in Biological Matrices"],"prefix":"10.1002","volume":"49","author":[{"given":"Xinyue","family":"Zheng","sequence":"first","affiliation":[{"name":"Central Hospital of Dalian University of Technology Dalian University of Technology  Dalian People's Republic of China"},{"name":"School of Chemical Engineering Ocean and Life Sciences Dalian University of Technology  Panjin People's Republic of 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