{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T17:20:19Z","timestamp":1783012819644,"version":"3.54.6"},"reference-count":42,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,4,14]],"date-time":"2026-04-14T00:00:00Z","timestamp":1776124800000},"content-version":"vor","delay-in-days":13,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T00:00:00Z","timestamp":1775001600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100007129","name":"Natural Science Foundation of Shandong Province","doi-asserted-by":"publisher","award":["ZR2023QB233"],"award-info":[{"award-number":["ZR2023QB233"]}],"id":[{"id":"10.13039\/501100007129","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2026,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    This study aimed to isolate and purify compounds from seven\u2010steamed and seven\u2010dried\n                    <jats:italic>Panax quinquefolius<\/jats:italic>\n                    L. by employing a novel separation strategy combining macroporous resin column chromatography, high\u2010speed countercurrent chromatography (HSCCC), and an on\u2010line storage inner\u2010recycling countercurrent chromatography (OS\u2010IRCCC) mode for the first time, which yielded 12 compounds, including 11 ginsenosides and one methyl naphthalene derivative. Steaming processing of\n                    <jats:italic>P. quinquefolius<\/jats:italic>\n                    triggers significant chemical transformations, characterized by the progressive degradation of major ginsenosides and the concomitant formation and marked accumulation of rare ginsenosides. After preliminary fractionation of the crude extract using a D101 macroporous resin column, three fractions (Fractions III, V, and VI) were selected for further targeted separation. Fractions III and VI were separated using the solvent system of dichloromethane\/methanol\/H\n                    <jats:sub>2<\/jats:sub>\n                    O\/isopropyl alcohol with different ratios of 6:5:4:2 and 6.2:4.8:4:2, respectively. For fraction V, traditional HSCCC combined with OS\u2010IRCCC technology was adopted, using methyl\n                    <jats:italic>tert<\/jats:italic>\n                    \u2010butyl ether\/\n                    <jats:italic>n<\/jats:italic>\n                    \u2010butanol\/acetonitrile\/H\n                    <jats:sub>2<\/jats:sub>\n                    O (4:2:3:7, v\/v). Ultimately, 12 compounds were obtained, including ginsenoside Rd (\n                    <jats:bold>1<\/jats:bold>\n                    , 6.2\u00a0mg), ginsenoside Re (\n                    <jats:bold>2<\/jats:bold>\n                    , 7.5\u00a0mg), ginsenoside Rg\n                    <jats:sub>1<\/jats:sub>\n                    (\n                    <jats:bold>3<\/jats:bold>\n                    , 2.3\u00a0mg), 20(\n                    <jats:italic>R<\/jats:italic>\n                    )\u2010ginsenoside Rh\n                    <jats:sub>1<\/jats:sub>\n                    (\n                    <jats:bold>4<\/jats:bold>\n                    , 8.3\u00a0mg), ginsenoside Rb\n                    <jats:sub>1<\/jats:sub>\n                    (\n                    <jats:bold>5<\/jats:bold>\n                    , 3.7\u00a0mg), rubilactone (\n                    <jats:bold>6<\/jats:bold>\n                    , 6.4\u00a0mg), ginsenoside Re\n                    <jats:sub>8<\/jats:sub>\n                    (\n                    <jats:bold>7<\/jats:bold>\n                    , 6.3\u00a0mg), 20(\n                    <jats:italic>S<\/jats:italic>\n                    )\u2010ginsenoside Rg\n                    <jats:sub>2<\/jats:sub>\n                    (\n                    <jats:bold>8<\/jats:bold>\n                    , 12.1\u00a0mg), 20(\n                    <jats:italic>R<\/jats:italic>\n                    )\u2010ginsenoside Rg\n                    <jats:sub>2<\/jats:sub>\n                    (\n                    <jats:bold>9<\/jats:bold>\n                    , 5.7\u00a0mg), damulin B (\n                    <jats:bold>10<\/jats:bold>\n                    , 2.7\u00a0mg), ginsenoside Rb\n                    <jats:sub>3<\/jats:sub>\n                    (\n                    <jats:bold>11<\/jats:bold>\n                    , 4.3\u00a0mg), and ginsenoside Rg\n                    <jats:sub>3<\/jats:sub>\n                    (\n                    <jats:bold>12<\/jats:bold>\n                    , 5.1\u00a0mg). This research highlights the efficacy of the novel separation technique for isolating and purifying valuable compounds from seven\u2010steamed and seven\u2010dried\n                    <jats:italic>P. quinquefolius<\/jats:italic>\n                    , providing a robust methodological framework for the targeted purification of complex natural products.\n                  <\/jats:p>","DOI":"10.1002\/jssc.70414","type":"journal-article","created":{"date-parts":[[2026,4,15]],"date-time":"2026-04-15T05:19:17Z","timestamp":1776230357000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["An Efficient Strategy to Separate the Main Ginsenosides From Steamed\n                    <i>Panax quinquefolius<\/i>\n                    by a Combination of Consecutive and Online\u2010Storage Inner\u2010Recycling High\u2010Speed Counter\u2010Current Chromatography"],"prefix":"10.1002","volume":"49","author":[{"given":"Longsheng","family":"Guo","sequence":"first","affiliation":[{"name":"College of Chemical Engineering Qingdao University of Science and Technology  Qingdao China"},{"name":"Chemical Technology Research Institute of Shandong Qingdao University of Science and Technology  Jinan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinqian","family":"Yu","sequence":"additional","affiliation":[{"name":"Shandong Analysis and Test Center School of Pharmaceutical Sciences Qilu University of Technology (Shandong Academy of Sciences)  Jinan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kai","family":"Zhong","sequence":"additional","affiliation":[{"name":"Shandong Analysis and Test Center School of Pharmaceutical Sciences Qilu University of Technology (Shandong Academy of Sciences)  Jinan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"Wang","sequence":"additional","affiliation":[{"name":"Shandong Analysis and Test Center School of Pharmaceutical Sciences Qilu University of Technology (Shandong Academy of Sciences)  Jinan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuning","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Chemical Engineering Qingdao University of Science and Technology  Qingdao China"},{"name":"Chemical Technology Research Institute of Shandong Qingdao University of Science and Technology  Jinan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yingjian","family":"Guo","sequence":"additional","affiliation":[{"name":"Shandong Analysis and Test Center School of Pharmaceutical Sciences Qilu University of Technology (Shandong Academy of Sciences)  Jinan 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