{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T23:39:56Z","timestamp":1792193996875,"version":"4.4.0"},"reference-count":38,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,4,24]],"date-time":"2026-04-24T00:00:00Z","timestamp":1776988800000},"content-version":"vor","delay-in-days":23,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T00:00:00Z","timestamp":1775001600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32402783"],"award-info":[{"award-number":["32402783"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2026,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The demand for sustainable methods to extract bioactive compounds from fruits is increasing, yet conventional extraction methods are limited by their reliance on large amounts of organic solvents, limiting their widespread application. Therefore, we evaluated a deep eutectic solvent (DES)\u2010ultrasound\u2010assisted extraction to efficiently extract phenolic compounds from\n                    <jats:italic>Lonicera caerulea<\/jats:italic>\n                    L. berries. Using single\u2010factor experiments and response surface methodology, the optimal conditions were determined: A DES composed of choline chloride and 1,3\u2010butanediol at a 1:5 molar ratio, with 29% water content, a solid\u2013liquid ratio of 1:41\u00a0g\/mL, and ultrasonication for 50\u00a0min. This method yielded 68.63 \u00b1 0.46\u00a0mg gallic acid\/g of total polyphenols, a 1.5\u2010fold increase over the conventional solvent (50% ethanol). Furthermore, UHPLC\u2010Q\u2010TOF\u2010MS\/MS analysis confirmed the presence of 31 phytochemicals in the extract, including cyanidin\u20103\u2010O\u2010glucoside, cyanidin\u20103\u2010O\u2010rutinoside, chlorogenic acid, procyanidin B2, rutin, and quercitrin, among others. Scanning electron microscopy analysis confirmed the synergistic mechanism of DES permeation and ultrasound\u2010induced cell wall disruption, and the mechanism of DES formation was studied by Fourier transform infrared spectroscopy. Further studies revealed that the extract exhibited significant antioxidant activity, with IC\n                    <jats:sub>50<\/jats:sub>\n                    values of 8.57 \u00b1 0.19 \u00b5g\/mL, 409.13 \u00b1 6.77 \u00b5g\/mL, and 123.17 \u00b1 3.25\u00a0\u00b5g\/mL for DPPH\u2022, \u2022OH, and ABTS\n                    <jats:sup>+<\/jats:sup>\n                    \u2022 assays, respectively, and demonstrated pancreatic lipase inhibition with an IC\n                    <jats:sub>50<\/jats:sub>\n                    of 4.31 \u00b1 0.12\u00a0mg\/mL. In the zebrafish model of non\u2010alcoholic fatty liver disease, the extract (200\u00a0\u00b5g\/mL) significantly reduced hepatic lipid accumulation, and decreased total cholesterol, triglycerides, alanine aminotransferase, and aspartate aminotransferase levels by 64%, 60%, 60%, and 55%, respectively, confirming potent lipid\u2010lowering and hepatoprotective efficacy. Overall, DES combined with ultrasound\u2010assisted extraction could serve as an eco\u2010friendly method for recovering high\u2010value compounds from fruits and their byproducts.\n                  <\/jats:p>","DOI":"10.1002\/jssc.70418","type":"journal-article","created":{"date-parts":[[2026,4,25]],"date-time":"2026-04-25T05:36:11Z","timestamp":1777095371000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Green and Efficient Extraction of Polyphenols From\n                    <i>Lonicera caerulea<\/i>\n                    L. Berries and Evaluation of Their Antioxidant and Lipid\u2010Lowering Activities"],"prefix":"10.1002","volume":"49","author":[{"given":"Peishi","family":"Feng","sequence":"first","affiliation":[{"name":"College of Pharmaceutical Science Zhejiang University of Technology  Hangzhou China"},{"name":"Zhejiang Provincial Key Laboratory of TCM For Innovative R &amp; D and Digital Intelligent Manufacturing of TCM Great Health Products  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qinglin","family":"Gui","sequence":"additional","affiliation":[{"name":"College of Pharmaceutical Science Zhejiang University of Technology  Hangzhou China"},{"name":"Zhejiang Provincial Key Laboratory of TCM For Innovative R &amp; D and Digital Intelligent Manufacturing of TCM Great Health Products  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiajia","family":"Yi","sequence":"additional","affiliation":[{"name":"College of Pharmaceutical Science Zhejiang University of Technology  Hangzhou China"},{"name":"Zhejiang Provincial Key Laboratory of TCM For Innovative R &amp; D and Digital Intelligent Manufacturing of TCM Great Health Products  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenyue","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Pharmaceutical Science Zhejiang University of Technology  Hangzhou China"},{"name":"Zhejiang Provincial Key Laboratory of TCM For Innovative R &amp; D and Digital Intelligent Manufacturing of TCM Great Health Products  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Feng","sequence":"additional","affiliation":[{"name":"College of Pharmaceutical Science Zhejiang University of Technology  Hangzhou China"},{"name":"Zhejiang Provincial Key Laboratory of TCM For Innovative R &amp; D and Digital Intelligent Manufacturing of TCM Great Health Products  Hangzhou 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