{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T17:20:18Z","timestamp":1783012818572,"version":"3.54.6"},"reference-count":23,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,4,21]],"date-time":"2026-04-21T00:00:00Z","timestamp":1776729600000},"content-version":"vor","delay-in-days":20,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T00:00:00Z","timestamp":1775001600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["537343248"],"award-info":[{"award-number":["537343248"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2026,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Compound\u2010specific stable carbon isotope analysis by one\u2010dimensional liquid chromatography\u2010isotope ratio mass spectrometry (LC\u2010IRMS) is fundamentally constrained by the requirement for fully aqueous mobile phases, which limits chromatographic selectivity and prevents effective separation of analytes from complex sample matrices. Here, a heart\u2010cutting two\u2010dimensional LC\u2010IRMS method (2D\u2010LC\u2010IRMS) is presented for the first compound\u2010specific\n                    <jats:italic>\u03b4<\/jats:italic>\n                    <jats:sup>13<\/jats:sup>\n                    C analysis of the water\u2010soluble vitamins B5 (pantothenic acid) and B9 (folic acid) in commercial dietary supplements and fortified beverages. An organic\u2010modified reversed\u2010phase separation in the first dimension achieved matrix reduction, while a fully aqueous second\u2010dimension separation ensured IRMS compatibility and delivered baseline\u2010resolved analyte peaks for precise isotope determination. Validation demonstrated linear calibration over 2\u2013100\u00a0mgC\u00a0L\n                    <jats:sup>\u22121<\/jats:sup>\n                    with\n                    <jats:italic>R<\/jats:italic>\n                    <jats:sup>2<\/jats:sup>\n                    \u2265\u00a00.9999, repeatability of \u2264\u00a00.13\u2030, and isotope\u2010stability\u2010based method detection limits of 5\u00a0mgC\u00a0L\n                    <jats:sup>\u22121<\/jats:sup>\n                    . Analyzed\n                    <jats:italic>\u03b4<\/jats:italic>\n                    <jats:sup>13<\/jats:sup>\n                    C values were independent of chromatographic configuration, confirming that heart\u2010cutting transfer does not introduce isotope fractionation. Application to 12 commercial products, including tablets, effervescent formulations, powdered supplements, and an energy drink, yielded\n                    <jats:italic>\u03b4<\/jats:italic>\n                    <jats:sup>13<\/jats:sup>\n                    C ranges of \u221220.6\u2030\u00a0to\u00a0\u221232.9\u2030 for vitamin B5 and \u221220.4\u2030\u00a0to\u00a0\u221236.4\u2030 for vitamin B9, reflecting differences in synthetic production routes across manufacturers. The presented workflow extends LC\u2010IRMS to the compound class of water soluble vitamins that were so far inaccessible to this technique and provides a broadly applicable strategy for compound\u2010specific isotope analysis of polar analytes in challenging matrices.\n                  <\/jats:p>","DOI":"10.1002\/jssc.70419","type":"journal-article","created":{"date-parts":[[2026,4,21]],"date-time":"2026-04-21T17:15:57Z","timestamp":1776791757000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Heart\u2010Cutting Two\u2010Dimensional Liquid Chromatography\u2010Isotope Ratio Mass Spectrometry for Compound\u2010Specific\n                    <i>\u03b4<\/i>\n                    <sup>13<\/sup>\n                    C Analysis of Water\u2010Soluble B Vitamins in Complex Supplement Matrices"],"prefix":"10.1002","volume":"49","author":[{"given":"Sarah P.","family":"Rockel","sequence":"first","affiliation":[{"name":"Instrumental Analytical Chemistry University of Duisburg\u2010Essen  Essen Germany"},{"name":"Centre for Water and Environmental Research (ZWU) University of Duisburg\u2010Essen  Essen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacqueline","family":"Martiny","sequence":"additional","affiliation":[{"name":"Instrumental Analytical Chemistry University of Duisburg\u2010Essen  Essen Germany"},{"name":"MDS Holding GmbH &amp; Co. KG  Dortmund Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maik A.","family":"Jochmann","sequence":"additional","affiliation":[{"name":"Instrumental Analytical Chemistry University of Duisburg\u2010Essen  Essen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Torsten C.","family":"Schmidt","sequence":"additional","affiliation":[{"name":"Instrumental Analytical Chemistry University of Duisburg\u2010Essen  Essen Germany"},{"name":"Centre for Water and Environmental Research (ZWU) University of Duisburg\u2010Essen  Essen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,4,21]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac203197d"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1080\/10643389.2021.1993053"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.trac.2024.117666"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/rcm.5167"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/mas.20149"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00216\u2010011\u20105683\u2010y"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1080\/10408398.2021.1997909"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.3390\/foods11213500"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11694\u2010025\u201003337\u2010z"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00216\u2010007\u20101309\u20109"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/jms.1190300311"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.4c05956"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.5c05840"},{"issue":"3","key":"e_1_2_9_15_1","first-page":"23","article-title":"Vitamin B\u2010Complex Deficiency, Supplementation and Management","volume":"86","author":"Schellack G.","year":"2019","journal-title":"Saudi Pharmaceutical Journal"},{"key":"e_1_2_9_16_1","unstructured":"Thermo Fisher Scientific Determination of Water\u2010 and Fat\u2010Soluble Vitamins by HPLC Technical Note 72488 (Thermo Fisher Scientific 2017)."},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00216\u2010020\u201002392\u20103"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/rcm.5069"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac300116w"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/rcm.2784"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.trac.2021.116515"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.1c05226"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10311\u2010006\u20100081\u2010y"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.trac.2016.11.008"}],"container-title":["Journal of Separation Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jssc.70419","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jssc.70419","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jssc.70419","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T16:40:45Z","timestamp":1783010445000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/jssc.70419"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,4]]},"references-count":23,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2026,4]]}},"alternative-id":["10.1002\/jssc.70419"],"URL":"https:\/\/doi.org\/10.1002\/jssc.70419","archive":["Portico"],"relation":{},"ISSN":["1615-9306","1615-9314"],"issn-type":[{"value":"1615-9306","type":"print"},{"value":"1615-9314","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,4]]},"assertion":[{"value":"2026-03-11","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-04-11","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-04-21","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70419"}}