{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T21:19:04Z","timestamp":1783459144122,"version":"3.55.0"},"reference-count":33,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,5,2]],"date-time":"2026-05-02T00:00:00Z","timestamp":1777680000000},"content-version":"vor","delay-in-days":1,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T00:00:00Z","timestamp":1777593600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2026,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Derrisisoflavone A is a prenylated isoflavonoid and a chemical marker in the stem of\n                    <jats:italic>Derris scandens<\/jats:italic>\n                    (Roxb.) Benth., it is a suitable chemical marker for this plant. Conventional analytical techniques, such as high\u2010performance liquid chromatography, liquid chromatography\u2013mass spectrometry, and immunoassays, offer high sensitivity but rely on complex analytical equipment and specialized expertise, thereby limiting their applicability for rapid on\u2010site analysis. In this study, a lateral flow immunochromatographic assay (LFIA) employing gold nanoparticle (AuNP)\u2010labeled monoclonal antibodies (mAbs) was developed for qualitative detection of derrisisoflavone A in\n                    <jats:italic>D. scandens<\/jats:italic>\n                    plant materials and herbal products. The LFIA was designed in a competitive format using AuNPs\u2010mAbs conjugates as the detection probe, derrisisoflavone A\u2010cationized ovalbumin conjugates applied on the test zone, and anti\u2010mouse immunoglobulin G antibody on the control zone. Under optimized conditions, the assay was evaluated for sensitivity and specificity and validated by comparison with a well\u2010established indirect competitive enzyme\u2010linked immunosorbent assay (icELISA) using various\n                    <jats:italic>D. scandens<\/jats:italic>\n                    plant parts, commercial products, and representative Fabaceae species. The LFIA exhibited a visual limit of detection of 100\u00a0\u00b5g\/mL, defined by complete inhibition of the test zone signal, and demonstrated high analytical specificity with undetectable cross\u2010reactivity toward structurally related isoflavonoids or prenylated flavonoids. The analytical results showed good agreement with those obtained by icELISA. Although less sensitive than quantitative methods, the developed LFIA provides a rapid, simple, and reliable screening tool for on\u2010site quality control and preliminary assessment of derrisisoflavone A in\n                    <jats:italic>D. scandens<\/jats:italic>\n                    and related products.\n                  <\/jats:p>","DOI":"10.1002\/jssc.70428","type":"journal-article","created":{"date-parts":[[2026,5,2]],"date-time":"2026-05-02T07:36:37Z","timestamp":1777707397000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A Monoclonal Antibody\u2010based Lateral Flow Immunochromatographic Assay for Rapid Detection of Derrisisoflavone A in\n                    <i>Derris scandens<\/i>\n                    (Roxb.) Benth"],"prefix":"10.1002","volume":"49","author":[{"given":"Pitragorn","family":"Srimongkon","sequence":"first","affiliation":[{"name":"Faculty of Pharmaceutical Sciences Khon Kaen University  Khon Kaen Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Worapol","family":"Sae\u2010Foo","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy and Pharmaceutical Botany Faculty of Pharmaceutical Sciences Prince of Songkla University  Hat Yai Thailand"},{"name":"Phytomedicine and Pharmaceutical Biotechnology Excellence Center Faculty of Pharmaceutical Sciences Prince of Songkla University  Hat Yai Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gorawit","family":"Yusakul","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Chemistry and Pharmacognosy Faculty of Pharmaceutical Sciences Naresuan University  Phitsanulok Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Waraporn","family":"Putalun","sequence":"additional","affiliation":[{"name":"Faculty of Pharmaceutical Sciences Khon Kaen University  Khon Kaen 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