{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T21:18:42Z","timestamp":1783459122343,"version":"3.55.0"},"reference-count":20,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,5,2]],"date-time":"2026-05-02T00:00:00Z","timestamp":1777680000000},"content-version":"vor","delay-in-days":1,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T00:00:00Z","timestamp":1777593600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2026,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    A micellar electrokinetic chromatography method was developed for the simultaneous separation of the phosphodiesterase\u20105 inhibitors sildenafil and tadalafil, two therapeutic agents used in the treatment of erectile dysfunction that are frequently identified as illicit adulterants in dietary supplements marketed for sexual enhancement. Method development was supported by a design of experiments strategy. An initial fractional factorial screening design was used to evaluate the influence of selected experimental parameters on resolution (R\n                    <jats:sub>s<\/jats:sub>\n                    ) and migration time. Pareto analysis of standardized effects indicated that cyclodextrin (CD) concentration, methanol (MeOH) content, and separation voltage significantly affected the separation, whereas pH, sodium dodecyl sulfate (SDS) concentration, and temperature did not show a statistically significant impact within the studied range. Based on the screening results, a Box\u2010Behnken response surface design was employed to optimize the significant factors. Optimized separation conditions consisted of a 50\u00a0mM phosphate buffer (pH 2.5) containing 50\u00a0mM SDS, 10% MeOH, and 5\u00a0mM hydroxypropyl\u2010\u03b2\u2010CD, with a separation voltage of \u201020\u00a0kV and a capillary temperature of 20\u00b0C. Under these conditions, baseline separation was achieved with a R\n                    <jats:sub>s<\/jats:sub>\n                    of 4.75 and migration times below 6\u00a0min. The method was validated in terms of precision, linearity, accuracy, and robustness, showing satisfactory analytical performance. Application to pharmaceutical formulations and dietary supplement samples confirmed the suitability of the proposed method for routine screening and quality control purposes.\n                  <\/jats:p>","DOI":"10.1002\/jssc.70429","type":"journal-article","created":{"date-parts":[[2026,5,2]],"date-time":"2026-05-02T07:38:31Z","timestamp":1777707511000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Design of Experiments\u2010assisted Micellar Electrokinetic Chromatography Separation of Phosphodiesterase\u20105 Inhibitors: Application to Sildenafil and Tadalafil"],"prefix":"10.1002","volume":"49","author":[{"given":"Cristina","family":"Nicula\u0219","sequence":"first","affiliation":[{"name":"Medicine and Pharmacy Doctoral School \"George Emil Palade\" University of Medicine, Pharmacy, Science and Technology of T\u00e2rgu Mure\u0219 T\u00e2rgu Mure\u0219 Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriel","family":"Hancu","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Chemistry Faculty of Pharmacy \u201cGeorge Emil Palade\u201d University of Medicine, Pharmacy, Science and Technology of T\u00e2rgu Mure\u0219 T\u00e2rgu Mure\u0219 Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zolt\u00e1n Istv\u00e1n","family":"Szab\u00f3","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Industry and Biotechnologies Faculty of Pharmacy \u201cGeorge Emil Palade\u201d University of Medicine, Pharmacy, Science and Technology of T\u00e2rgu Mure\u0219 T\u00e2rgu Mure\u0219 Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anca","family":"Gabriela C\u00e2rje","sequence":"additional","affiliation":[{"name":"Department of Analytical Chemistry and Pharmaceutical Analysis Faculty of Pharmacy \u201cGeorge Emil Palade\u201d University of Medicine, Pharmacy, Science and Technology of T\u00e2rgu Mure\u0219 T\u00e2rgu Mure\u0219 Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valentin","family":"Ion","sequence":"additional","affiliation":[{"name":"Department of Analytical Chemistry and Pharmaceutical Analysis Faculty of Pharmacy \u201cGeorge Emil Palade\u201d University of Medicine, Pharmacy, Science and Technology of T\u00e2rgu Mure\u0219 T\u00e2rgu Mure\u0219 Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniela Lucia","family":"Muntean","sequence":"additional","affiliation":[{"name":"Department of Analytical Chemistry and Pharmaceutical Analysis Faculty of Pharmacy \u201cGeorge Emil Palade\u201d University of Medicine, Pharmacy, Science and Technology of T\u00e2rgu Mure\u0219 T\u00e2rgu Mure\u0219 Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,5,2]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ucl.2021.06.009"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2021.735708"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.2147\/ciia.2006.1.4.403"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.2147\/tcrm.s3336"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cofs.2018.10.012"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/1541\u20104337.12173"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.3390\/pharmacy11050154"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcph.2046"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.3390\/ph17091119"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2004.07.016"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1039\/C3AY26470J"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/1522\u20102683(200106)22"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021\u20109673(02)00131\u20100"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/elps.200290028"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1365\/s10337\u2010004\u20100366\u2010x"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2014.08.045"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.microc.2025.115037"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02292944"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1590\/s2175\u201097902018000001006"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.0c01887"}],"container-title":["Journal of Separation Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jssc.70429","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jssc.70429","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jssc.70429","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T20:51:55Z","timestamp":1783457515000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/jssc.70429"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,5]]},"references-count":20,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2026,5]]}},"alternative-id":["10.1002\/jssc.70429"],"URL":"https:\/\/doi.org\/10.1002\/jssc.70429","archive":["Portico"],"relation":{},"ISSN":["1615-9306","1615-9314"],"issn-type":[{"value":"1615-9306","type":"print"},{"value":"1615-9314","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,5]]},"assertion":[{"value":"2026-03-04","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-04-21","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-05-02","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70429"}}