{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T21:19:00Z","timestamp":1783459140592,"version":"3.55.0"},"reference-count":26,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,5,2]],"date-time":"2026-05-02T00:00:00Z","timestamp":1777680000000},"content-version":"vor","delay-in-days":1,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T00:00:00Z","timestamp":1777593600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81873085"],"award-info":[{"award-number":["81873085"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81403183"],"award-info":[{"award-number":["81403183"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2026,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Recently, bile acids (BAs) have garnered significant attention due to their crucial roles in various metabolic diseases. However, the simultaneous quantification of BAs in biological samples is challenging due to the structural similarities, complex biological matrix, and tissue\u2010specific distribution. We developed a sensitive UHPLC\u2013MS\/MS method for quantitation of 19 BAs in mouse liver (mainly conjugated BAs) and feces (mainly unconjugated BAs). We compared protein precipitation, liquid\u2013liquid extraction, and solid\u2010phase extraction (SPE) methods for sample preparation, and SPE was chosen due to the low background noise and high extraction efficiency for both conjugated and unconjugated BAs. The method was extensively validated by evaluating the linearity (\n                    <jats:italic>R<\/jats:italic>\n                    <jats:sup>2<\/jats:sup>\n                    \u00a0\u2265\u00a00.991), extraction recovery (82%\u2013112%), limits of detection (1\u20132\u00a0ng\/mL) and low limits of quantification (2\u20135\u00a0ng\/mL). Then, the method was applied to the detection of BAs in liver and feces of metabolic dysfunction\u2010associated steatotic liver disease mice, and the correlations between BA levels and the mRNA expression levels of BA metabolic enzymes, transporters, and receptors were analyzed. This study provides a direct and reliable determination method for the in\u2010depth investigation of the enterohepatic circulation of BAs.\n                  <\/jats:p>","DOI":"10.1002\/jssc.70430","type":"journal-article","created":{"date-parts":[[2026,5,2]],"date-time":"2026-05-02T07:34:43Z","timestamp":1777707283000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Development and Validation of a Sensitive UHPLC\u2013MS\/MS Method for the Simultaneous Determination of 19 Bile Acids in Liver and Feces: Application to Metabolic Dysfunction\u2010Associated Steatotic Liver Disease Mice"],"prefix":"10.1002","volume":"49","author":[{"given":"Meng","family":"Xiao","sequence":"first","affiliation":[{"name":"School of Traditional Chinese Medicine Capital Medical University  Beijing China"},{"name":"National Institutes for Food and Drug Control  Beijing 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