{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,13]],"date-time":"2026-09-13T02:48:55Z","timestamp":1789267735646,"version":"build-2803163510"},"reference-count":39,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,5,10]],"date-time":"2026-05-10T00:00:00Z","timestamp":1778371200000},"content-version":"vor","delay-in-days":9,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T00:00:00Z","timestamp":1777593600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2026,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Glycopeptide enrichment is an essential prerequisite in mass spectrometry (MS)\u2010based protein glycosylation studies, due to the low abundance of glycosylated peptides and the high complexity of biological samples, which contain substantial interference from non\u2010glycosylated metabolites. Current enrichment strategies commonly exploit the polar interactions between glycans and hydrophilic solid\u2010phase materials. Building on existing magnetic\u2010core particles, we developed a series of novel magnetic materials with surfaces functionalized with dextran and amide groups. The magnetic core, composed of polyvinylpyrrolidone (PVP) and Fe\n                    <jats:sub>3<\/jats:sub>\n                    O\n                    <jats:sub>4<\/jats:sub>\n                    , was sequentially coated with SiO\n                    <jats:sub>2<\/jats:sub>\n                    and dextran. Amide groups were then conjugated to the dextran layer via a condensation reaction between carboxy\u2010modified dextran and ethanolamine. The glycopeptide enrichment performance of five resulting magnetic materials was evaluated using IgG and rat serum samples. One material, designated Fe\n                    <jats:sub>3<\/jats:sub>\n                    O\n                    <jats:sub>4<\/jats:sub>\n                    @SiO\n                    <jats:sub>2<\/jats:sub>\n                    @Dextran\u2010Amide (Dextran\u2010polyamide\u2010717), demonstrated superior sensitivity for glycopeptide detection and strong resistance to nonspecific interference. Moreover, Dextran\u2010polyamide\u2010717 exhibited higher enrichment efficiency in both IgG and serum samples compared to two commercially available products. With its multilayer surface modification strategy, Dextran\u2010polyamide\u2010717 shows great promise for advanced applications in glycoproteomic research.\n                  <\/jats:p>","DOI":"10.1002\/jssc.70445","type":"journal-article","created":{"date-parts":[[2026,5,11]],"date-time":"2026-05-11T05:53:41Z","timestamp":1778478821000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Synthesis and Validation of Dextran\u2010Based Hydrophilic Magnetic Nanomaterials for Glycopeptide Enrichment"],"prefix":"10.1002","volume":"49","author":[{"given":"Li","family":"Cheng","sequence":"first","affiliation":[{"name":"Wisdom Lake Academy of Pharmacy Xi'an Jiaotong\u2010Liverpool University  Suzhou China"},{"name":"XJTLU Wisdom Lake Academy of Pharmacy\u2010BEAVER Biomedical Joint Laboratory  Suzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qirui","family":"Fu","sequence":"additional","affiliation":[{"name":"Wisdom Lake Academy of Pharmacy Xi'an Jiaotong\u2010Liverpool University  Suzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingxian","family":"Huang","sequence":"additional","affiliation":[{"name":"XJTLU Wisdom Lake Academy of Pharmacy\u2010BEAVER Biomedical Joint Laboratory  Suzhou China"},{"name":"BEAVER Laboratories  Suzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"Ren","sequence":"additional","affiliation":[{"name":"XJTLU Wisdom Lake Academy of Pharmacy\u2010BEAVER Biomedical Joint Laboratory  Suzhou China"},{"name":"BEAVER Laboratories  Suzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yiqiang","family":"Wang","sequence":"additional","affiliation":[{"name":"Wisdom Lake Academy of Pharmacy Xi'an Jiaotong\u2010Liverpool University  Suzhou China"},{"name":"XJTLU Wisdom Lake Academy of Pharmacy\u2010BEAVER Biomedical Joint Laboratory  Suzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingming","family":"Xu","sequence":"additional","affiliation":[{"name":"Wisdom Lake Academy of Pharmacy Xi'an Jiaotong\u2010Liverpool University  Suzhou China"},{"name":"XJTLU Wisdom Lake Academy of Pharmacy\u2010BEAVER Biomedical Joint Laboratory  Suzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,5,10]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.200501023"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/database\/baab012"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41392\u2010024\u201001886\u20101"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10337\u2010025\u201004407\u20106"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.2c00524"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/glycob\/cwv091"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.201000517"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00216\u2010009\u20102664\u20105"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10719\u2010012\u20109376\u20103"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/mas.20265"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/9780470027318.a9309.pub2"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2005.01.030"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/mas.20200"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/mas.20283"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0304\u20104165(99)00165\u20108"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.trac.2018.10.009"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt827"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/0968\u20100004(93)90193\u2010Q"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00712a019"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/0021\u20109673(92)85457\u20105"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.M600390\u2010MCP200"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1006983125913"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021\u20109673(00)96972\u20103"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jproteome.6b00098"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00216\u2010024\u201005708\u20109"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.200600199"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.201200434"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aca.2013.11.049"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010017\u201007516\u2010x"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1039\/c3cc45008b"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1039\/c2cc33600f"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.3390\/antib9020022"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00216\u2010025\u201005995\u2010w"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aca.2019.11.012"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.8b00461"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.202100466"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1039\/C4NR05955G"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1021\/acssuschemeng.8b06171"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aca.2012.10.002"}],"container-title":["Journal of Separation Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jssc.70445","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jssc.70445","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jssc.70445","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T20:51:37Z","timestamp":1783457497000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/jssc.70445"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,5]]},"references-count":39,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2026,5]]}},"alternative-id":["10.1002\/jssc.70445"],"URL":"https:\/\/doi.org\/10.1002\/jssc.70445","archive":["Portico"],"relation":{},"ISSN":["1615-9306","1615-9314"],"issn-type":[{"value":"1615-9306","type":"print"},{"value":"1615-9314","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,5]]},"assertion":[{"value":"2025-09-25","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-05-05","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-05-10","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70445"}}