{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T21:19:04Z","timestamp":1783459144129,"version":"3.55.0"},"reference-count":36,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,5,24]],"date-time":"2026-05-24T00:00:00Z","timestamp":1779580800000},"content-version":"vor","delay-in-days":23,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T00:00:00Z","timestamp":1777593600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2026,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    In this study, a series of oligomer\u2010brush chiral stationary phases was developed to systematically investigate the influence of amino acid side chains on enantioseparation. Through a structure\u2010preserving \u201cgrafting\u2010to\u201d strategy, three well\u2010defined chiral oligomers derived from\n                    <jats:sc>l\u2010<\/jats:sc>\n                    proline,\n                    <jats:sc>l\u2010<\/jats:sc>\n                    phenylalanine, and\n                    <jats:sc>l\u2010<\/jats:sc>\n                    leucine were covalently immobilized onto silica microspheres. The enantioseparation capabilities of these chiral stationary phases were comprehensively evaluated using 27 representative racemates under normal\u2010phase conditions. The results revealed that the side\u2010chain architecture plays a decisive role in the chiral recognition microenvironment. The stationary phase with a rigid pyrrolidine ring (\n                    <jats:bold>CSP\u2010P(Pro\u2010An)<\/jats:bold>\n                    ) demonstrated the broadest applicability for bulky, conformationally restricted analytes and was the only stationary phase capable of resolving the tested axially chiral metal complexes. The stationary phase with aromatic side chains (\n                    <jats:bold>CSP\u2010P(Phe\u2010An)<\/jats:bold>\n                    ) showed enhanced selectivity for specific\n                    <jats:italic>\u03c0<\/jats:italic>\n                    \u2010rich analytes, while the stationary phase with a flexible isobutyl side chain (\n                    <jats:bold>CSP\u2010P(Leu\u2010An)<\/jats:bold>\n                    ) provided the highest separation factors for several 3,5\u2010dinitrobenzoyl\u2010derivatized amino acids. Furthermore, an investigation into the influence of mobile\u2010phase modifiers found that, compared to other aprotic modifiers such as ethyl acetate, tetrahydrofuran, and dichloromethane, methyl\n                    <jats:italic>tert<\/jats:italic>\n                    \u2010butyl ether provided a more optimal balance between retention and enantioselectivity. This is attributed to the steric shielding effect produced by the\n                    <jats:italic>tert<\/jats:italic>\n                    \u2010butyl group of methyl\n                    <jats:italic>tert<\/jats:italic>\n                    \u2010butyl ether, which minimizes its competitive interactions for essential hydrogen\u2010bonding sites. These results indicate that both the side\u2010chain structure and the choice of mobile\u2010phase modifier are key parameters for tailoring the performance of amino acid\u2010based oligomer\u2010brush chiral stationary phases.\n                  <\/jats:p>","DOI":"10.1002\/jssc.70453","type":"journal-article","created":{"date-parts":[[2026,5,25]],"date-time":"2026-05-25T06:17:33Z","timestamp":1779689853000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Amino Acid Oligomer Brushes as Chiral Stationary Phases: Effects of Side\u2010Chain Architecture and Mobile\u2010Phase Modifiers on Enantioseparation Behavior"],"prefix":"10.1002","volume":"49","author":[{"given":"Changyu","family":"Cai","sequence":"first","affiliation":[{"name":"Engineering Research Center of Pharmaceutical Process Chemistry Ministry of Education School of Pharmacy East China University of Science and Technology  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingming","family":"Cao","sequence":"additional","affiliation":[{"name":"Engineering Research Center of Pharmaceutical Process Chemistry Ministry of Education School of Pharmacy East China University of Science and Technology  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beilei","family":"Zhang","sequence":"additional","affiliation":[{"name":"Engineering Research Center of Pharmaceutical Process Chemistry Ministry of Education School of Pharmacy East China University of Science and Technology  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5412-2869","authenticated-orcid":false,"given":"Yanxiong","family":"Ke","sequence":"additional","affiliation":[{"name":"Engineering Research Center of Pharmaceutical Process Chemistry Ministry of Education School of Pharmacy East China University of Science and Technology  Shanghai 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