{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T21:18:49Z","timestamp":1783459129573,"version":"3.55.0"},"reference-count":55,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,5,23]],"date-time":"2026-05-23T00:00:00Z","timestamp":1779494400000},"content-version":"vor","delay-in-days":22,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T00:00:00Z","timestamp":1777593600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2022YFD2200805"],"award-info":[{"award-number":["2022YFD2200805"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2026,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    An integrated and eco\u2010friendly method for the simultaneous extraction and enrichment of camptothecin (CPT) alkaloids and hyperoside (HYP), including CPT, 10\u2010hydroxycamptothecin (HCPT), 10\u2010methoxycamptothecin (MCPT), and HYP from\n                    <jats:italic>Camptotheca acuminata<\/jats:italic>\n                    seeds, was developed using mixed\u2010micelle extraction and enrichment by cloud point property (MME\u2010ECP). A mixed\u2010surfactant system, optimally formulated with 4% Triton X\u2010114 and 4% Genapol X\u2010080, demonstrated superior extraction efficiency for the four target compounds when synergistically combined with ultrasonic assistance. The cloud point behavior of the surfactants facilitated the enrichment of the four compounds. Various experimental conditions were investigated to evaluate and optimize the extraction and preconcentration process. Under optimal conditions, the extraction yields of CPT, HCPT, MCPT, and HYP reached 1007.62, 1408.61, 268.45, and 157.67 \u00b5g\/g, respectively. Compared to traditional methanol\/ethanol extraction, the mixed\u2010micelle method demonstrates superior performance, especially over ethanol extraction, with an extraction yield enhancement of 40.20%\u2013168.81%. Furthermore, the surfactants were successfully recycled from the extraction medium using AB\u20108 macroporous adsorption resin, achieving high recovery rates of 83.32% for T\u2010114 and 87.36% for GX\u2010080. Overall, this study highlights the integrated MME\u2010ECP method as an efficient, economical, and eco\u2010friendly approach for extracting and enriching the key active compounds from\n                    <jats:italic>C. acuminata<\/jats:italic>\n                    seeds. This method holds great promise as an innovative extraction technique, with considerable potential for large\u2010scale industrial use.\n                  <\/jats:p>","DOI":"10.1002\/jssc.70454","type":"journal-article","created":{"date-parts":[[2026,5,23]],"date-time":"2026-05-23T11:49:29Z","timestamp":1779536969000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Synergistic Mixed\u2010Micelle Extraction Coupled With Cloud Point Enrichment for Simultaneous Recovery of Camptothecin Alkaloids and Hyperoside From\n                    <i>Camptotheca acuminata<\/i>\n                    Seeds"],"prefix":"10.1002","volume":"49","author":[{"given":"Jing","family":"Zhou","sequence":"first","affiliation":[{"name":"Key Laboratory of Forest Plant Ecology\u2010Ministry of Education Engineering Research Center of Forest Bio\u2010Preparation\u2010Ministry of Education Heilongjiang Provincial Key Laboratory of Ecological Utilization of Forestry\u2010based active Compounds College of Chemistry Chemical Engineering and Resource Utilization Northeast Forestry University  Harbin P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Baoyu","family":"Qu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Forest Plant Ecology\u2010Ministry of Education Engineering Research Center of Forest Bio\u2010Preparation\u2010Ministry of Education Heilongjiang Provincial Key Laboratory of Ecological Utilization of Forestry\u2010based active Compounds College of Chemistry Chemical Engineering and Resource Utilization Northeast Forestry University  Harbin P. R. 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