{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T10:29:10Z","timestamp":1786616950201,"version":"build-2736575974"},"reference-count":32,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81473322"],"award-info":[{"award-number":["81473322"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>A rapid and reliable ultra\u2010high\u2010performance liquid chromatography\u2010quadrupole time\u2010of\u2010flight mass spectrometry (UHPLC\u2013Q\u2010TOF\u2010MS) strategy was established to characterize the chemical constituents of Qing'e Pills in vitro and in vivo. In vitro profiling identified 32 compounds in the Psoraleae Fructus (PF) extract, and 30 compounds related to PF were characterized in the Qing'e Pills extract, mainly belonging to coumarins, dihydroflavones, isoflavones, and chalcones. Following a single oral administration of Qing'e Pills to healthy volunteers, nine prototype compounds were detectable in human serum, confirming systemic exposure to multiple constituents. For quantitative evaluation, a sensitive and selective UHPLC\u2013triple quadrupole\u2010tandem MS (UHPLC\u2013QqQ\u2010MS\/MS) method was developed and fully validated for simultaneous determination of five representative analytes (psoralen, isopsoralen [IP], bavachin, neobavaisoflavone, and corylin) in human serum, with osalmid as the internal standard. The validated method was applied to a pharmacokinetic (PK) study in 12 female volunteers receiving Qing'e Pills at the Chinese Pharmacopeia\u2010recommended dose (9\u00a0g). Among the monitored analytes, only IP met the quantitative criteria and yielded a complete concentration\u2013time profile, indicating low circulating levels of the remaining components at this clinically relevant dose. Isopsoralen was rapidly absorbed (detectable within 0.5\u00a0h), reached a maximum concentration of 85.47 \u00b1 28.96\u00a0ng\/mL at 1.5\u00a0h, and exhibited an area under the curve of 619.52 \u00b1 160.25 ng\u00b7h\/mL with a terminal half\u2010life of 5.06 \u00b1 0.28\u00a0h, suggesting limited systemic exposure with moderate elimination. Collectively, this validated UHPLC\u2013QqQ\u2010MS\/MS platform provides a practical approach for chemical profiling and human PK evaluation of coumarins and flavonoids from Qing'e Pills.<\/jats:p>","DOI":"10.1002\/jssc.70475","type":"journal-article","created":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T06:55:40Z","timestamp":1782975340000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Pharmacokinetic Study of Five Lead Components of Psoraleae Fructus in Human Serum by UHPLC\u2013Q\u2010TOF\u2010MS and UHPLC\u2013QqQ\u2010MS\/MS after Oral Administration of Qing'e Pills"],"prefix":"10.1002","volume":"49","author":[{"given":"Zhuoqing","family":"Li","sequence":"first","affiliation":[{"name":"State Key Laboratory of Discovery and Utilization of Functional Components in Traditional Chinese Medicine and the MOE Key Laboratory for Standardization of Chinese Medicines Shanghai University of Traditional Chinese Medicine  Shanghai China"},{"name":"School of Traditional Chinese Pharmacy China Pharmaceutical University  Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jieling","family":"Su","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Discovery and Utilization of Functional Components in Traditional Chinese Medicine and the MOE Key Laboratory for Standardization of Chinese Medicines Shanghai University of Traditional Chinese Medicine  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaokun","family":"Xu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Discovery and Utilization of Functional Components in Traditional Chinese Medicine and the MOE Key Laboratory for Standardization of Chinese Medicines Shanghai University of Traditional Chinese Medicine  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinyin","family":"Cai","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Discovery and Utilization of Functional Components in Traditional Chinese Medicine and the MOE Key Laboratory for Standardization of Chinese Medicines Shanghai University of Traditional Chinese Medicine  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kan","family":"Zhong","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Discovery and Utilization of Functional Components in Traditional Chinese Medicine and the MOE Key Laboratory for Standardization of Chinese Medicines Shanghai University of Traditional Chinese Medicine  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qingshan","family":"Zheng","sequence":"additional","affiliation":[{"name":"Center For Drug Clinical Research Shanghai University of Traditional Chinese Medicine  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Yang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Discovery and Utilization of Functional Components in Traditional Chinese Medicine and the MOE Key Laboratory for Standardization of Chinese Medicines Shanghai University of Traditional Chinese Medicine  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhengtao","family":"Wang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Discovery and Utilization of Functional Components in Traditional Chinese Medicine and the MOE Key Laboratory for Standardization of Chinese Medicines Shanghai University of Traditional Chinese Medicine  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lujin","family":"Li","sequence":"additional","affiliation":[{"name":"Center For Drug Clinical Research Shanghai University of Traditional Chinese Medicine  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zijia","family":"Zhang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Discovery and Utilization of Functional Components in Traditional Chinese Medicine and the MOE Key Laboratory for Standardization of Chinese Medicines Shanghai University of Traditional Chinese Medicine  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,7,1]]},"reference":[{"key":"e_1_2_11_2_1","first-page":"48","article-title":"Qing'e Pills (Heji Ju Fang)","volume":"6","author":"Hong W. 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