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To reduce nonspecific interactions between analytes and the inner surface of the separation compartment and then improve detection sensitivity and separation efficiency, it is essential to modify fused silica capillaries with functional groups. In this study, we synthesized a zwitterionic antifouling peptide (Glu\u2010Lys)\n                    <jats:sub>3<\/jats:sub>\n                    Glu using solid\u2010phase peptide synthesis. Then, we bound the peptide to the inner surface of a fused silica capillary using diazo resin as a coupling agent, resulting in stable and excellent antifouling and separation abilities. To evaluate the properties of the coating, we conducted fluorescent protein adsorption and electrophoresis experiments. The obtained results demonstrated that the coating effectively prevented protein adsorption and made it possible to separate five standard proteins in 20\u00a0min. Moreover, three proteins of egg white were separated within 15\u00a0min. These findings confirmed the very good anti\u2010protein adsorption performance of the peptide\u2010coated capillary and its practical value for various applications.\n                  <\/jats:p>","DOI":"10.1002\/jssc.70479","type":"journal-article","created":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T07:09:47Z","timestamp":1782976187000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Covalent Zwitterionic Peptide\u2010Based Antifouling Coating of the Fused Silica Capillary Applied for CE Separation of Proteins"],"prefix":"10.1002","volume":"49","author":[{"given":"Qinghua","family":"Cao","sequence":"first","affiliation":[{"name":"College of Chemistry and Chemical Engineering College of Materials Science and Engineering Institute of Biomedical Materials and Engineering Qingdao University  Qingdao 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