{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T18:02:01Z","timestamp":1789495321812,"version":"build-2803163510"},"reference-count":43,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2026,7,10]],"date-time":"2026-07-10T00:00:00Z","timestamp":1783641600000},"content-version":"vor","delay-in-days":9,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["22174129"],"award-info":[{"award-number":["22174129"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Pyrrolizidine alkaloids (PAs) and their N\u2010oxides (PANOs) are naturally occurring toxic plant metabolites that have attracted widespread attention due to their potential food safety hazards. In this study, an efficient analytical method for determining PAs\/PANOs in bee pollen was established by combining accelerated solvent extraction (ASE) with ultra\u2010high performance liquid chromatography\u2010tandem mass spectrometry (UHPLC\u2010MS\/MS). A magnetic covalent organic framework material (Fe\n                    <jats:sub>3<\/jats:sub>\n                    O\n                    <jats:sub>4<\/jats:sub>\n                    @COF\u2010TAPB\u2010TFP) was synthesized and used as an adsorbent for offline purification to reduce matrix interference and improve detection sensitivity. Under optimal conditions, the method showed excellent linearity (\n                    <jats:italic>R<\/jats:italic>\n                    <jats:sup>2<\/jats:sup>\n                    \u2265 0.99) in the range of 1\u2013200 \u00b5g\u00b7L\n                    <jats:sup>\u2212<\/jats:sup>\n                    <jats:sup>1<\/jats:sup>\n                    , with recoveries of 70.1%\u201395.6% for spiked blank samples at three levels (10, 50, and 100 \u00b5g\u00b7kg\n                    <jats:sup>\u2212<\/jats:sup>\n                    <jats:sup>1<\/jats:sup>\n                    ), relative standard deviations below 15%, and limits of quantification of 1.2\u20139.9 \u00b5g\u00b7kg\n                    <jats:sup>\u2212<\/jats:sup>\n                    <jats:sup>1<\/jats:sup>\n                    . This method was successfully applied to the analysis of local market bee pollen samples, with detected PAs\/PANOs levels ranging from 25.6 to 178.6 \u00b5g\u00b7kg\n                    <jats:sup>\u2212<\/jats:sup>\n                    <jats:sup>1<\/jats:sup>\n                    . Overall, the Fe\n                    <jats:sub>3<\/jats:sub>\n                    O\n                    <jats:sub>4<\/jats:sub>\n                    @COF\u2010TAPB\u2010TP\u2010assisted ASE\u2010UHPLC\u2010MS\/MS method provides an accurate, rapid, highly automated, and high\u2010throughput platform for monitoring PAs\/PANOs contamination in food products.\n                  <\/jats:p>","DOI":"10.1002\/jssc.70488","type":"journal-article","created":{"date-parts":[[2026,7,10]],"date-time":"2026-07-10T12:38:12Z","timestamp":1783687092000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Determination of Pyrrolizidine Alkaloids and Their N\u2011Oxides in Bee Pollen by Accelerated Solvent Extraction Followed by Magnetic Covalent Organic Framework\u2010Based Offline Purification and Ultra\u2010High\u2010Performance Liquid Chromatography\u2010Tandem Mass Spectrometry"],"prefix":"10.1002","volume":"49","author":[{"given":"Yuhang","family":"Wang","sequence":"first","affiliation":[{"name":"College of Chemical Engineering Zhejiang University of Technology  Hangzhou China"},{"name":"Key Laboratory of Pollution Exposure and Health Intervention of Zhejiang Province, Interdisciplinary Research Academy Zhejiang Shuren University  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yujia","family":"Guo","sequence":"additional","affiliation":[{"name":"Key Laboratory of Pollution Exposure and Health Intervention of Zhejiang Province, Interdisciplinary Research Academy Zhejiang Shuren University  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guoda","family":"Wang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Pollution Exposure and Health Intervention of Zhejiang Province, Interdisciplinary Research Academy Zhejiang Shuren University  Hangzhou 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