{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T05:19:18Z","timestamp":1787807958901,"version":"build-2784847793"},"reference-count":29,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2026,8,11]],"date-time":"2026-08-11T00:00:00Z","timestamp":1786406400000},"content-version":"vor","delay-in-days":10,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Crataegi Folium (CF), a traditional Chinese medicine widely used for promoting blood circulation and regulating lipid metabolism, contains diverse bioactive constituents with potential health\u2010promoting effects. However, the rapid localization and identification of its functional compounds remain challenging due to the complexity of its chemical constituents. This study aimed to establish an integrated approach using high\u2010performance thin\u2010layer chromatography (HPTLC) coupled with bioactivity assay and mass spectrometry (MS) for the rapid screening and identification of pancreatic lipase inhibitory and antibacterial constituents in CF. Two HPTLC developing systems with different polarity ranges were optimized to comprehensively separate the chemical constituents, enabling effect\u2010directed analysis. The results revealed that the pancreatic lipase inhibitory activity originated primarily from low\u2010polarity triterpenoids. One active zone, consisting of co\u2010migrating isomers, was successfully separated by in\u2010situ iodine derivatization and characterized as maslinic acid and corosolic acid through comparison with reference standards. Another active zone was identified as arjunolic acid by using the exclusion method. Based on the\n                    <jats:italic>Vibrio fischeri<\/jats:italic>\n                    bioassay, six observed\n                    <jats:italic>V. fischeri<\/jats:italic>\n                    bioluminescence\u2010inhibitory zones were mainly associated with high\u2010polarity compounds, among which five were identified as isoquercitrin, hyperoside, chlorogenic acid, vitexin\u20102\u2032\u2032\u2010O\u2010rhamnoside, and vitexin\u20104\u2032\u2032\u2010O\u2010glucoside. Our findings provide new insights into bioactive constituents of CF, and demonstrate that HPTLC\u2010bioautography\u2010MS is an efficient and integrated platform for the rapid discovery and identification of markers for quality assessment of bioactive compounds in complex botanical matrices.\n                  <\/jats:p>","DOI":"10.1002\/jssc.70497","type":"journal-article","created":{"date-parts":[[2026,8,11]],"date-time":"2026-08-11T09:39:57Z","timestamp":1786441197000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Discovery of Pancreatic Lipase Inhibitory and Antimicrobial Constituents in Crataegi Folium by High\u2010Performance Thin\u2010Layer Chromatography\u2010Bioautography\u2010Mass Spectrometry"],"prefix":"10.1002","volume":"49","author":[{"given":"Liang","family":"Zhu","sequence":"first","affiliation":[{"name":"State Key Laboratory of Discovery and Utilization of Functional Components in Traditional Chinese Medicine The Ministry of Education (MOE) Key Laboratory for Standardization of Chinese Medicine  Shanghai China"},{"name":"Shanghai Key Laboratory of Compound Chinese Medicine Institute of Chinese Materia Medica Shanghai University of Traditional Chinese Medicine  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"An\u2010Lu","family":"Ma","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Discovery and Utilization of Functional Components in Traditional Chinese Medicine The Ministry of Education (MOE) Key Laboratory for Standardization of Chinese Medicine  Shanghai China"},{"name":"Shanghai Key Laboratory of Compound Chinese Medicine Institute of Chinese Materia Medica Shanghai University of Traditional Chinese Medicine  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi\u2010Lian","family":"Zhang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Discovery and Utilization 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