{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T05:19:10Z","timestamp":1787807950171,"version":"build-2784847793"},"reference-count":44,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T00:00:00Z","timestamp":1786579200000},"content-version":"vor","delay-in-days":12,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Swertia purpurascens<\/jats:italic>\n                    is an important ethno\u2010medicinal herb used for treating fever and colds. Despite its significance, the phytochemical profile of this species remains unexplored. This study aims to conduct a phytochemical investigation, develop a green and reliable analytical method assessing 11 specialized metabolites of\n                    <jats:italic>S. purpurascens<\/jats:italic>\n                    , and identify their suitable biological targets via network pharmacology. Among isolated metabolites, 3\u2010hydroxybenzyl\u2010\u03b2\u2010\n                    <jats:sc>d<\/jats:sc>\n                    \u2010glucopyranoside (\n                    <jats:bold>1<\/jats:bold>\n                    ) has been reported for the first time from the\n                    <jats:italic>Swertia<\/jats:italic>\n                    genus, whereas compounds gentiopicroside (\n                    <jats:bold>2<\/jats:bold>\n                    ), 1\u2010hydroxy\u20103,5\u2010dimethoxyxanthone (\n                    <jats:bold>5<\/jats:bold>\n                    ), and methylswertianin (\n                    <jats:bold>6<\/jats:bold>\n                    ) have been isolated for the first time from\n                    <jats:italic>S. purpurascens<\/jats:italic>\n                    . The developed method showed good linearity, sensitivity, precision, accuracy, and a positive green analytical profile. Results revealed that the stem part was the major source of metabolites (Yield: 125.70\u00a0\u00b1\u00a01.79\u00a0mg\/g), compared with the other analyzed parts (flowers, leaves, and roots). Compounds\n                    <jats:bold>2<\/jats:bold>\n                    (0.72%\u20136.94%),\n                    <jats:bold>9<\/jats:bold>\n                    (0.08%\u20135.61%), and\n                    <jats:bold>1<\/jats:bold>\n                    (0.07%\u20132.71%) were quantified as the major compounds among different plant parts. Furthermore, network pharmacology analysis predicted the potential therapeutic relevance of these compounds with suggested pathways related to diabetes and cancer diseases. This study represents the first attempt to simultaneously quantify 11 specialized metabolites of\n                    <jats:italic>S. purpurascens<\/jats:italic>\n                    and to perform a network pharmacology\u2010based analysis, thereby enriching its phytochemical understanding.\n                  <\/jats:p>","DOI":"10.1002\/jssc.70498","type":"journal-article","created":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T10:58:45Z","timestamp":1786618725000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Quantitative Analysis and Network Pharmacology\u2010Based Profiling of Isolated Metabolites from\n                    <i>Swertia purpurascens<\/i>\n                    (D.Don) C.B. Clarke"],"prefix":"10.1002","volume":"49","author":[{"given":"Km","family":"Swati","sequence":"first","affiliation":[{"name":"Chemical Technology Division CSIR\u2010Institute of Himalayan Bioresource Technology  Palampur India"},{"name":"Academy of Scientific and Innovative Research (AcSIR)  Ghaziabad India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nitin","family":"Kumar","sequence":"additional","affiliation":[{"name":"Chemical Technology Division CSIR\u2010Institute of Himalayan Bioresource Technology  Palampur India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Taniya","family":"Sharma","sequence":"additional","affiliation":[{"name":"Chemical Technology Division CSIR\u2010Institute of Himalayan Bioresource Technology  Palampur India"},{"name":"Academy of Scientific and Innovative Research (AcSIR)  Ghaziabad India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pooja","family":"Bhatt","sequence":"additional","affiliation":[{"name":"Chemical Technology Division CSIR\u2010Institute of Himalayan Bioresource Technology  Palampur India"},{"name":"Academy of Scientific and Innovative Research (AcSIR)  Ghaziabad India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pamita","family":"Bhandari","sequence":"additional","affiliation":[{"name":"Chemical Technology Division CSIR\u2010Institute of Himalayan Bioresource Technology  Palampur India"},{"name":"Academy of Scientific and Innovative Research (AcSIR)  Ghaziabad India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,13]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-819541-3.00019-0"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1142\/S0192415x17500380"},{"key":"e_1_2_10_4_1","first-page":"1","article-title":"Swertia L. (Gentianaceae) in Nepal: Ethnobotany and Agenda for Sustainable Management","volume":"2008","author":"Joshi K.","year":"2008","journal-title":"Ethnobotanical Leaflets"},{"key":"e_1_2_10_5_1","first-page":"191","article-title":"Medicinal Ethnic Botany Research of Swertia Genus","volume":"27","author":"Liu T.","year":"2015","journal-title":"Natural Product Research and Development"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.sajb.2019.05.030"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.indcrop.2019.111569"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbdv.200490123"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-981-15-1394-7_4"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.3923\/crc.2011.1.15"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2015.00308"},{"key":"e_1_2_10_12_1","first-page":"785","article-title":"A Review Article on Chirayata (Genus Swertia)","volume":"10","author":"Raj D.","year":"2021","journal-title":"World Journal of Pharmaceutical Research"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/jps.2600630827"},{"key":"e_1_2_10_14_1","first-page":"482","article-title":"Comparative Morphological Studies of Some Swertia Species","volume":"6","author":"Mehta A.","year":"2017","journal-title":"Journal of Pharmacognosy and Phytochemistry"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jics.2025.101781"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.talanta.2021.123046"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.0c01887"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1039\/D1GC02318G"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-801814-9.00005-2"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.3390\/ph15050572"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1381\u20101177(01)00073\u2010X"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules22112002"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1055\/s\u20102006\u2010959560"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1515\/znb\u20102002\u20100115"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/pca.1362"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/0031\u20109422(91)80080\u2010K"},{"key":"e_1_2_10_27_1","unstructured":"ICH\u2010Guideline ICH Harmonised Tripartite Validation of Analytical Procedures: Text and Methodology Q2 (R1) 1(ICH 2005)."},{"key":"e_1_2_10_28_1","volume-title":"Bioanalytical Method Validation and Study Sample Analysis M10","author":"ICH Guideline, I. H","year":"2022"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.3390\/foods14193438"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers14215268"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2015.12.006"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2018.00606"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yfrne.2013.07.005"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1097\/WOX.0b013e3182439613"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1002\/ar.21146"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2014.00018"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrd2467"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.3390\/metabo8010019"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1440\u20101681.2011.05599.x"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/s1043\u20102760(00)00272\u20101"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.2174\/1568010023344571"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers6020897"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbdv.202402306"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.2147\/DDDT.S299753"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2021.114741"}],"container-title":["Journal of Separation Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jssc.70498","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jssc.70498","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jssc.70498","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T04:38:23Z","timestamp":1787805503000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/jssc.70498"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8]]},"references-count":44,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2026,8]]}},"alternative-id":["10.1002\/jssc.70498"],"URL":"https:\/\/doi.org\/10.1002\/jssc.70498","archive":["Portico"],"relation":{},"ISSN":["1615-9306","1615-9314"],"issn-type":[{"value":"1615-9306","type":"print"},{"value":"1615-9314","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8]]},"assertion":[{"value":"2026-02-25","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-07-14","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-13","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70498"}}