{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T05:18:48Z","timestamp":1787807928726,"version":"build-2784847793"},"reference-count":76,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T00:00:00Z","timestamp":1787184000000},"content-version":"vor","delay-in-days":19,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["#303952\/2024\u20105"],"award-info":[{"award-number":["#303952\/2024\u20105"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["#04607\/2019\u20103"],"award-info":[{"award-number":["#04607\/2019\u20103"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Mangiferin (MGF), a bioactive C\u2010glucosylxanthone predominantly found in\n                    <jats:italic>Mangifera indica<\/jats:italic>\n                    L., has attracted increasing interest due to its recognized antioxidant and pharmacological properties. In this study, an aqueous ethanolic maceration process was systematically optimized for MGF recovery from mango plant biomass using a Taguchi experimental design followed by Box\u2010Behnken response surface methodology. The optimized conditions of 39.9\u00b0C, 8\u00a0h, and 80% v\/v ethanol yielded 41.60\u00a0mg\/g dry weight of MGF, confirming the robustness of the predictive model. Biochemical profiling of the optimized extract revealed 4.36\u00a0mg gallic acid equivalents\/g dry weight of total phenolics, 73.12\u00a0mg\/g of reducing sugars, and low protein content, indicating a phytochemical matrix enriched in glycosylated phenolics. Antioxidant evaluation by the 2,2\u2010diphenyl\u20101\u2010picrylhydrazyl radical scavenging assay demonstrated 87.74% radical inhibition at 400\u00a0\u00b5g\/mL and a 50% inhibitory concentration of 225\u00a0\u00b5g\/mL, reflecting strong radical scavenging capacity. Greenness assessment using the Analytical GREEnness metric approach software yielded a score of 0.72, validating the environmental compatibility of the proposed method. The results demonstrate that statistically guided aqueous ethanolic maceration represents an efficient, scalable, and sustainable platform for the recovery of MGF\u2010rich extracts with potential pharmaceutical, nutraceutical, and cosmetic applications.\n                  <\/jats:p>","DOI":"10.1002\/jssc.70500","type":"journal-article","created":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T16:18:51Z","timestamp":1787242731000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Sustainable Extraction of Mangiferin From Mango Leaves Using Taguchi and Box\u2010Behnken Optimization: Biochemical Characterization and Antioxidant Profiling"],"prefix":"10.1002","volume":"49","author":[{"given":"Astrid","family":"Corrales","sequence":"first","affiliation":[{"name":"Bioseparations and Nanoformulations Laboratory (BIOSEP NANOLAB) Department of Biotechnology Lorena School of Engineering University of S\u00e3o Paulo (DEBIQ, EEL\/USP) Lorena Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vitor de P\u00e1dua","family":"Alves","sequence":"additional","affiliation":[{"name":"Lorena Technical School (COTEL) \u2013 Prof. Nelson Pesciotta \u2013 Lorena School of Engineering University of S\u00e3o Paulo  Lorena Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00falia Naelly Machado","family":"Silva","sequence":"additional","affiliation":[{"name":"Natural Products Chemistry Laboratory Institute of Chemistry University of S\u00e3o Paulo (LQPN, IQ\/USP) S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Felipe Rebello","family":"Louren\u00e7o","sequence":"additional","affiliation":[{"name":"Department of Faculty of Pharmaceutical Sciences LabMEQ \u2010 Laboratory of Metrology, Examinology, and Quality by Design University of S\u00e3o Paulo S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Massuo Jorge","family":"Kato","sequence":"additional","affiliation":[{"name":"Natural Products Chemistry Laboratory Institute of Chemistry University of S\u00e3o Paulo (LQPN, IQ\/USP) S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andr\u00e9 Moreni","family":"Lopes","sequence":"additional","affiliation":[{"name":"Bioseparations and Nanoformulations Laboratory (BIOSEP NANOLAB) Department of Biotechnology Lorena School of Engineering University of S\u00e3o Paulo (DEBIQ, EEL\/USP) Lorena Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,20]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/life13122270"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/9781119534167.ch6"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.2147\/NSA.S525423"},{"key":"e_1_2_9_5_1","unstructured":"ChemSpider \u201cMangiferin \u201dhttps:\/\/www.chemspider.com\/Chemical\u2010Structure.4444966.html CSID: 4444966 Accessed: Mar 13 2026. (2025)."},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.31488\/jjm.1000109"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.2174\/1389557511313030011"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1517\/13543776.2013.836182"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.2174\/138955712800493870"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/biof.1693"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.4155\/ppa\u20102016\u20100003"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.3892\/mmr.2021.12112"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.3390\/plants12071539"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1080\/01496395.2025.2452411"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1080\/10408347.2025.2503437"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.70198"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.70325"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.201300518"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1080\/10408347.2023.2197495"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1039\/D4GC02512A"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.seppur.2025.136174"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.seppur.2020.117757"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.202300925"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.coche.2025.101120"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/adfm.201500877"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/fsn3.3869"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.3390\/pr9050852"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1080\/10408398.2021.1983767"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1039\/D4GC05977H"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1111\/jfpe.70079"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40204\u2010018\u20100099\u20109"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13399\u2010022\u201002550\u2010w"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tifs.2021.11.019"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0924\u20100136(98)00079\u2010X"},{"key":"e_1_2_9_35_1","first-page":"774","article-title":"Dilian Ramdana Ismukada, Antidiabetic Activity of Papaya Leaf Extract (Carica papaya L.) Isolated With Maceration Method in Alloxan Induces Diabetic Mice","volume":"11","author":"Solikhah T. I.","year":"2020","journal-title":"Systematic Reviews in Pharmacy"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules19010438"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0076\u20106879(99)99017\u20101"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003\u20102697(76)90527\u20103"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac60147a030"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0023\u20106438(95)80008\u20105"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.0c01887"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.seppur.2025.136031"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules19021411"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.indcrop.2019.111703"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2016.10.053"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13197\u2010014\u20101514\u20104"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.iecr.2c04305"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1039\/D1GC03662A"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cep.2023.109644"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-822139-6.00002-X"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13399\u2010023\u201004726\u20104"},{"key":"e_1_2_9_52_1","doi-asserted-by":"publisher","DOI":"10.1080\/10408398.2023.2201834"},{"key":"e_1_2_9_53_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.chemrev.1c00438"},{"key":"e_1_2_9_54_1","doi-asserted-by":"publisher","DOI":"10.3390\/antiox11071313"},{"key":"e_1_2_9_55_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf011064b"},{"key":"e_1_2_9_56_1","doi-asserted-by":"publisher","DOI":"10.1039\/C6OB01622G"},{"key":"e_1_2_9_57_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.indcrop.2017.10.057"},{"key":"e_1_2_9_58_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf800738r"},{"key":"e_1_2_9_59_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2019.01073"},{"key":"e_1_2_9_60_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ifset.2015.04.006"},{"key":"e_1_2_9_61_1","doi-asserted-by":"publisher","DOI":"10.4067\/S0717\u201075182014000300013"},{"key":"e_1_2_9_62_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-408117-8.00019-2"},{"key":"e_1_2_9_63_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijerph18020741"},{"key":"e_1_2_9_64_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2006.10.017"},{"key":"e_1_2_9_65_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fbio.2022.101783"},{"issue":"4","key":"e_1_2_9_66_1","first-page":"224","article-title":"Determination of Change in Sugar Content in Healthy and Diseased Leaves of Two Mango Varieties (Langra and Chaunsa) Affected With Quick Decline Disease","volume":"7","author":"Shaheen R.","year":"2015","journal-title":"Journal of Toxicological Sciences"},{"key":"e_1_2_9_67_1","doi-asserted-by":"publisher","DOI":"10.56588\/iabcd.v1i2.35"},{"key":"e_1_2_9_68_1","doi-asserted-by":"publisher","DOI":"10.1155\/jamc\/3469361"},{"key":"e_1_2_9_69_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-319-67625-8_17"},{"key":"e_1_2_9_70_1","doi-asserted-by":"publisher","DOI":"10.1080\/10408398.2021.1888693"},{"key":"e_1_2_9_71_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-981-19-6426-8_11"},{"key":"e_1_2_9_72_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nfs.2021.02.001"},{"key":"e_1_2_9_73_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fbp.2021.03.002"},{"key":"e_1_2_9_74_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chmed.2019.12.002"},{"key":"e_1_2_9_75_1","doi-asserted-by":"publisher","DOI":"10.3390\/pr11061767"},{"key":"e_1_2_9_76_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.microc.2023.108766"},{"key":"e_1_2_9_77_1","doi-asserted-by":"publisher","DOI":"10.3390\/separations9060147"}],"container-title":["Journal of Separation Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jssc.70500","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jssc.70500","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jssc.70500","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T04:38:54Z","timestamp":1787805534000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/jssc.70500"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8]]},"references-count":76,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2026,8]]}},"alternative-id":["10.1002\/jssc.70500"],"URL":"https:\/\/doi.org\/10.1002\/jssc.70500","archive":["Portico"],"relation":{},"ISSN":["1615-9306","1615-9314"],"issn-type":[{"value":"1615-9306","type":"print"},{"value":"1615-9314","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8]]},"assertion":[{"value":"2026-03-31","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-07-28","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-20","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70500"}}