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(\n                    <jats:italic>Ligusticum chuanxiong<\/jats:italic>\n                    Hort. or\n                    <jats:italic>Ligusticum wallichii<\/jats:italic>\n                    ) is a traditional medicinal herb in East Asia used for cardiovascular, neurological, and inflammatory disorders, but developmental phytochemical changes remain unclear. Here, targeted liquid chromatography\u2010tandem mass spectrometry (LC\u2010MS\/MS) and untargeted headspace solid\u2010phase microextraction with gas chromatography\u2010MS were applied to examine five bioactive compounds and volatile organic compounds (VOCs) in aerial and root tissues across seven growth periods. Five compounds, including ferulic acid, icariside F2, ligusticoside A,\n                    <jats:italic>N<\/jats:italic>\n                    \u20103\u2010(3\u2010methoxy\u20104\u2010hydroxyphenyl) allyl pipecolic acid, and ligustiphenol, were quantified with excellent linearity, sensitivity, precision, and accuracy. VOC profiling identified 33 and 32 compounds in aerial parts and roots, respectively, showing clear period\u2010 and tissue\u2010specific differences. Chemometrics analysis revealed distinct clustering by growth stage, indicating dynamic metabolic shifts. Overall, roots served as reservoirs of phenolic acids, while aerial parts were enriched in phthalides and sesquiterpenes. These findings provide a basis for harvest optimization, quality control, and standardization of\n                    <jats:italic>L. striatum<\/jats:italic>\n                    , while novel compounds in Vietnamese herbal medicines expand its phytochemical diversity and pharmacological potential.\n                  <\/jats:p>","DOI":"10.1002\/jssc.70502","type":"journal-article","created":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T06:08:36Z","timestamp":1787033316000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Integrated Liquid Chromatography\u2010Tandem Mass Spectrometry and Headspace Solid\u2010Phase Microextraction With Gas Chromatography\u2010Mass Spectrometry\u2010based Profiling Reveals Developmental Metabolic Dynamics of\n                    <i>Ligusticum striatum<\/i>\n                    DC."],"prefix":"10.1002","volume":"49","author":[{"given":"Ninh Khac Thanh","family":"Tung","sequence":"first","affiliation":[{"name":"College of Pharmacy Chungnam National University  Daejeon South Korea"},{"name":"Institute of Chemistry Vietnam Academy of Science and Technology  Hanoi Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nguyen Hoai","family":"Nam","sequence":"additional","affiliation":[{"name":"Institute of Chemistry Vietnam Academy of Science and Technology  Hanoi Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nguyen Xuan","family":"Nhiem","sequence":"additional","affiliation":[{"name":"Institute of Chemistry Vietnam Academy of Science and Technology  Hanoi Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nguyen Tien","family":"Dat","sequence":"additional","affiliation":[{"name":"Center for High Technology Research and Development Vietnam Academy of Science and Technology  Hanoi Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sung Won","family":"Kwon","sequence":"additional","affiliation":[{"name":"College of Pharmacy Seoul National University  Seoul South Korea"},{"name":"Natural Products Research Institute Seoul National University  Seoul South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jong Seong","family":"Kang","sequence":"additional","affiliation":[{"name":"College of Pharmacy Chungnam National University  Daejeon South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hyung Min","family":"Kim","sequence":"additional","affiliation":[{"name":"College of Pharmacy Chungnam National University  Daejeon South 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