{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T10:56:10Z","timestamp":1790592970080,"version":"4.1.0"},"reference-count":25,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2026,9,13]],"date-time":"2026-09-13T00:00:00Z","timestamp":1789257600000},"content-version":"vor","delay-in-days":12,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Deschloroketamine (DXE) is a dissociative new psychoactive substance whose enantiomers and metabolites may exhibit distinct pharmacological and toxicological profiles. Reliable analytical methods enabling the chiral determination of DXE and its metabolites in biological matrices are thus essential. In this study, we evaluated supercritical fluid chromatography coupled to tandem mass spectrometry (SFC\u2011MS\/MS) and capillary zone electrophoresis with ultraviolet detection (CZE\u2011UV) for the enantioselective analysis of DXE and its major metabolites in rat brain tissue. SFC using polysaccharide\u2011based chiral stationary phases provided baseline separation of the hydroxylated and demethylated metabolites, but severe peak distortion of DXE enantiomers prevented their quantification. Therefore, quantitative analysis was performed by CZE employing carboxymethyl\u2011\u03b2\u2011cyclodextrin as a chiral selector and a high\u2011sensitivity Z\u2011shaped detection cell. Analysis of brain tissue samples obtained following high\u2010dose administration revealed near equimolar concentrations of the DXE enantiomers and a higher concentration of (\n                    <jats:italic>R<\/jats:italic>\n                    )\u2010norDXE relative to (\n                    <jats:italic>S<\/jats:italic>\n                    )\u2010norDXE. In this particular case, the combined use of SFC\u2011MS\/MS and CZE\u2011UV provided a practical workflow for the chiral profiling of DXE and its metabolites in complex biological matrices.\n                  <\/jats:p>","DOI":"10.1002\/jssc.70503","type":"journal-article","created":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T05:45:19Z","timestamp":1789364719000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Determination of Enantiomers of Deschloroketamine and Its Metabolites in Wistar Rat Brains"],"prefix":"10.1002","volume":"49","author":[{"given":"Natalie","family":"Pa\u0161kanov\u00e1","sequence":"first","affiliation":[{"name":"Forensic Laboratory of Biologically Active Substances Department of Chemistry of Natural Compounds University of Chemistry and Technology Prague  Prague 6 Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vendulka","family":"Hrub\u00e1","sequence":"additional","affiliation":[{"name":"Department of Analytical Chemistry Faculty of Chemical Engineering University of Chemistry and Technology Prague  Prague 6 Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Fr\u00fchauf","sequence":"additional","affiliation":[{"name":"Department of Analytical Chemistry University of Vienna  Vienna Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bronislav","family":"Jur\u00e1sek","sequence":"additional","affiliation":[{"name":"Forensic Laboratory of Biologically Active Substances Department of Chemistry of Natural Compounds University of Chemistry and Technology Prague  Prague 6 Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"Kucha\u0159","sequence":"additional","affiliation":[{"name":"Forensic Laboratory of Biologically Active Substances Department of Chemistry of Natural Compounds University of Chemistry and Technology Prague  Prague 6 Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pavel","family":"\u0158ezanka","sequence":"additional","affiliation":[{"name":"Department of Analytical Chemistry Faculty of Chemical Engineering University of Chemistry and Technology Prague  Prague 6 Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michal","family":"Kohout","sequence":"additional","affiliation":[{"name":"Department of Organic Chemistry University of Chemistry and Technology Prague  Prague 6 Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,13]]},"reference":[{"key":"e_1_2_10_2_1","unstructured":"D.PreissandA.Tatar \u201cUse of 2\u2010Methylamino\u20102\u2010Phenylcyclohexanone for the Treatment of Bacterial Fungal Viral or Protozoal Infections and for Immunomodulation \u201d US Patent: US5811464A (1995)."},{"key":"e_1_2_10_3_1","unstructured":"C. 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