{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T05:19:59Z","timestamp":1787807999650,"version":"build-2784847793"},"reference-count":26,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T00:00:00Z","timestamp":1786665600000},"content-version":"vor","delay-in-days":13,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100004281","name":"Narodowe Centrum Nauki","doi-asserted-by":"publisher","award":["2024\/08\/X\/NZ7\/00637"],"award-info":[{"award-number":["2024\/08\/X\/NZ7\/00637"]}],"id":[{"id":"10.13039\/501100004281","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Direct oral anticoagulants (DOACs), including apixaban, dabigatran, edoxaban, and rivaroxaban, may require quantitative measurement in selected clinical situations despite fixed\u2010dose administration. This study developed and validated a quick, easy, cheap, effective, rugged, and safe (QuEChERS)\u2010assisted liquid chromatography\u2010tandem mass spectrometry method for their simultaneous determination in citrate plasma and quantitative dried plasma spot (qDPS) samples. Chromatographic separation was achieved on a Kinetex PS C18 column using a 4.0 min gradient, followed by positive electrospray ionization and scheduled multiple reaction monitoring. Sample preparation was optimized by comparing acetonitrile (ACN) protein precipitation, unbuffered and citrate\u2010buffered QuEChERS extraction salts, and dispersive solid\u2010phase extraction clean\u2010up using primary\u2010secondary amine, C18, EMR Lipid, Z\u2010Sep+, or their combinations. The final procedure comprised ACN extraction with MgSO\n                    <jats:sub>4<\/jats:sub>\n                    , NaCl, and Bond Elut EMR Lipid, followed by clean\u2010up with MgSO\n                    <jats:sub>4<\/jats:sub>\n                    , primary\u2010secondary amine, and Z\u2010Sep+. ACN provided the most consistent recovery across analytes, concentration levels, and matrices. The method was validated in accordance with International Council for Harmonization M10 using commercial plasma calibrators and independently prepared quality\u2010control samples. Accuracy and precision met the predefined acceptance criteria in both matrices. Mean recovery ranged from 84.2% to 91.2% in citrate plasma and from 79.6% to 87.8% in dried plasma samples. Internal standard\u2010normalized matrix factors ranged from 0.87 to 0.98, indicating limited ion suppression after the optimized clean\u2010up procedure. External verification using materials from two independent proficiency\u2010testing providers showed satisfactory agreement with assigned target values, with all results falling within the providers\u2019 acceptance ranges. The method provides a robust QuEChERS\u2010assisted workflow for the quantitative determination of DOACs in conventional citrate plasma and qDPS samples. Its clinical interchangeability between matrices requires further evaluation using paired patient samples.\n                  <\/jats:p>","DOI":"10.1002\/jssc.70505","type":"journal-article","created":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T12:57:35Z","timestamp":1786712255000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Development and Validation of a QuEChERS\u2013LC\u2013MS\/MS Method for Simultaneous Determination of Four Direct Oral Anticoagulants in Citrate Plasma and Quantitative Dried Plasma Spots"],"prefix":"10.1002","volume":"49","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4833-8532","authenticated-orcid":false,"given":"Arkadiusz","family":"Kocur","sequence":"first","affiliation":[{"name":"Department of Drug Chemistry Pharmaceutical and Biomedical Analysis Faculty of Pharmacy Medical University of Warsaw  Warsaw 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