{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T15:24:26Z","timestamp":1790609066435,"version":"4.1.0"},"reference-count":19,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T00:00:00Z","timestamp":1787097600000},"content-version":"vor","delay-in-days":18,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Huangqi Shengmai Yin (HQSMY) displays notable cardioprotective properties. Nevertheless, the principal pharmacodynamic constituents underpinning this efficacy have not yet been fully clarified. To tackle this issue, an integrative workflow coupling UHPLC\u2011Q\u2011TOF\u2011MS\/MS fingerprinting, chemometrics, and spectrum\u2011effect correlations was constructed for the systematic discovery of cardioprotective markers. Fifty\u2010one components from 10 batches of HQSMY samples were characterized by UHPLC\u2011Q\u2011TOF\u2011MS\/MS in conjunction with the GNPS molecular networking platform. Subsequently, chromatographic fingerprints were established, with 22 and 29 characteristic common peaks assigned in negative and positive ion modes, respectively. Interbatch consistency was assessed via chemometric methods. Spectrum\u2011effect relationship analyses were conducted through Pearson correlation, gray relational analysis, and OPLS regression, revealing that formononetin, astrapterocarpan, astragaloside II, schisandrin, and biochanin A exhibited highly significant positive correlations with H9c2 cardiomyocyte protective activity. All these compounds attained gray relational scores exceeding 0.7, implying strong associations with cardioprotective action. These five components were then chosen from the candidate markers for subsequent functional validation using the CCK\u20108 assay, which yielded EC\n                    <jats:sub>50<\/jats:sub>\n                    values of 18.60 \u00b1 3.15 \u00b5M (biochanin A), 26.42 \u00b1 4.78 \u00b5M (formononetin), 92.93 \u00b1 19.14 \u00b5M (astragaloside II), 70.85 \u00b1 13.26 \u00b5M (schisandrin), and 72.73 \u00b1 14.05 \u00b5M (astrapterocarpan). The cardioprotective effect of astragaloside II, schisandrin, and astrapterocarpan in H\n                    <jats:sub>2<\/jats:sub>\n                    O\n                    <jats:sub>2<\/jats:sub>\n                    \u2010induced H9c2 injury model were reported for the first time. This integrated analytical strategy not only pinpoints the primary cardioprotective components of HQSMY but also furnishes a dependable methodological framework for quality evaluation and pharmacodynamic material research.\n                  <\/jats:p>","DOI":"10.1002\/jssc.70509","type":"journal-article","created":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T09:43:49Z","timestamp":1787132629000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Discovery of Cardioprotective Markers in Huangqi Shengmai Yin via Integrated UHPLC\u2010Q\u2010TOF\u2010MS\/MS Fingerprinting, Chemometric Quality Assessment, and Bioactivity Correlation"],"prefix":"10.1002","volume":"49","author":[{"given":"Peijing","family":"Jiang","sequence":"first","affiliation":[{"name":"Department of Pharmacy Yongkang First People's Hospital Affiliated to Hangzhou Medical College  Yongkang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yacheng","family":"Lv","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Yongkang First People's Hospital Affiliated to Hangzhou Medical College  Yongkang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jie","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Pharmaceutical Science Zhejiang University of Technology  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yangsheng","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Yongkang First People's Hospital Affiliated to Hangzhou Medical College  Yongkang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhuoying","family":"Jin","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Yongkang First People's Hospital Affiliated to Hangzhou Medical College  Yongkang 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