{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T05:18:53Z","timestamp":1787807933227,"version":"build-2784847793"},"reference-count":42,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T00:00:00Z","timestamp":1787616000000},"content-version":"vor","delay-in-days":24,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Natural bear bile powder is a precious traditional Chinese medicine that has a long history of use in clinical practice. The current study analyzed four batches of natural bear bile powder samples (\n                    <jats:bold>B\u20101\u223cB\u20104<\/jats:bold>\n                    ) using standards\u2010aided ultra\u2010high\u2010performance liquid chromatography\u2010quadrupole\u2010Orbitrap\u2010mass spectrometry and ultra\u2010performance liquid chromatography coupled with electrospray\u2010ionization quadrupole time\u2010of\u2010flight mass spectrometry. A total of 37 compounds (\n                    <jats:bold>1\u201037<\/jats:bold>\n                    ) were accurately identified, while 30 \u201cunreported\u201d compounds were found in natural bear bile powder for the first time. In particular, two \u201cunreported\u201d isomers (2'\u2010hydroxydaidzein and genistein) were strictly distinguished by means of \u0394R.T. matching in chromatographic stick\u2010peak and MS\/MS peak matching. The quantification work indicated that taurochenodeoxycholic acid (T\n                    <jats:styled-content>C<\/jats:styled-content>\n                    DCA) and its epimer tauroursodeoxycholic acid (T\n                    <jats:styled-content>U<\/jats:styled-content>\n                    DCA) had the highest contents in natural bear bile powder. However, T\n                    <jats:styled-content>C<\/jats:styled-content>\n                    DCA showed different binding energy towards pregnane X receptor from T\n                    <jats:styled-content>U<\/jats:styled-content>\n                    DCA in the molecular docking simulation. Based on the analytical chemistry, molecular docking simulation, and documentary evidence, T\n                    <jats:styled-content>C<\/jats:styled-content>\n                    DCA was recommended as a potential Q\u2010marker for pharmacopoeia. The new Q\u2010marker was validated to be feasible by analysis of 11 batches of natural bear bile powder samples (\n                    <jats:bold>B\u20105\u223cB\u201015<\/jats:bold>\n                    ), and the specific ratio of T\n                    <jats:styled-content>C<\/jats:styled-content>\n                    DCA\/T\n                    <jats:styled-content>U<\/jats:styled-content>\n                    DCA (0.58\u223c0.98) can distinguish natural BBP from artificial BBP and other animal biles. All these findings not only lay a foundation for manufacturing technique improvement of artificial natural bear bile powder, but also promote pharmacopoeia to update the quality\u2010control method.\n                  <\/jats:p>","DOI":"10.1002\/jssc.70513","type":"journal-article","created":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T08:35:14Z","timestamp":1787646914000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Two Standards\u2010Aided LC\u2010MS Technologies Uncover 30 \u201cUnreported\u201d Compounds in Natural Bear Bile Powder to Add a New Pharmacopoeia Quality\u2010Marker"],"prefix":"10.1002","volume":"49","author":[{"given":"Ziqing","family":"Li","sequence":"first","affiliation":[{"name":"School of Chinese Herbal Medicine Guangzhou University of Chinese Medicine  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haoyu","family":"Huang","sequence":"additional","affiliation":[{"name":"School of Chinese Herbal Medicine Guangzhou University of Chinese Medicine  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heping","family":"Zheng","sequence":"additional","affiliation":[{"name":"Key Laboratory of Functional Molecular Engineering of Guangdong Province School of Chemistry and Chemical Engineering South China University of Technology  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gongchang","family":"Zheng","sequence":"additional","affiliation":[{"name":"Key Laboratory of Functional Molecular Engineering of Guangdong Province School of Chemistry and Chemical Engineering South China University of Technology  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xi","family":"Zhao","sequence":"additional","affiliation":[{"name":"School of Chinese Herbal Medicine Guangzhou University of Chinese Medicine  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongbai","family":"Liang","sequence":"additional","affiliation":[{"name":"School of Chinese Herbal Medicine Guangzhou University of Chinese Medicine  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rongxin","family":"Cai","sequence":"additional","affiliation":[{"name":"Center of Pharmaceutical Preparation Qinghai Provincial Hospital of Traditional Chinese Medicine Xining China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hanxiao","family":"Chai","sequence":"additional","affiliation":[{"name":"School of Chinese Herbal Medicine Guangzhou University of Chinese Medicine  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongwei","family":"Song","sequence":"additional","affiliation":[{"name":"School of Chinese Herbal Medicine Guangzhou University of Chinese Medicine  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xican","family":"Li","sequence":"additional","affiliation":[{"name":"School of Chinese Herbal Medicine Guangzhou University of Chinese Medicine  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,25]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.eng.2023.09.017"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12934\u2010024\u201002592\u2010x"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mce.2012.05.004"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/s1875\u20105364(22)60169\u20109"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11655\u2010019\u20103010\u20101"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00216\u2010023\u201004740\u20105"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2022.115063"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/rcm.9326"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.3390\/separations9110329"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s44442\u2010025\u201000013\u20101"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2024.116222"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jafc.2c06563"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/pca.3543"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules29235764"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.microc.2023.108938"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/open.202400046"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11306\u2010007\u20100082\u20102"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2020.126760"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.talanta.2025.128867"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1039\/c3ay41605d"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.11669\/cpj.2016.22.014"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbdv.202201109"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.13260\/j.cnki.jfjtcm.2023.03008"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.7501\/j.issn.0253\u20102670.2018.01.001"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40643\u2010022\u201000517\u20103"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/adsc.202000120"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00216\u2010024\u201005553\u2010w"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsomega.4c08260"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.1002\/bmc.4835"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.isci.2025.113781"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.1111\/jfbc.13866"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2025.1515976"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.1039\/c8ra06798h"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbih.2023.100674"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018\u2010022\u201004278\u20102"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamem.2013.05.007"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1440\u20101746.1996.tb00290.x"},{"key":"e_1_2_11_39_1","doi-asserted-by":"publisher","DOI":"10.3892\/etm.2010.178"},{"key":"e_1_2_11_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.measurement.2016.03.022"},{"key":"e_1_2_11_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2010.10.116"},{"key":"e_1_2_11_42_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00216\u2010023\u201004791\u20108"},{"key":"e_1_2_11_43_1","doi-asserted-by":"publisher","DOI":"10.1080\/10408398.2021.1946006"}],"container-title":["Journal of Separation Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jssc.70513","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jssc.70513","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jssc.70513","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T04:39:37Z","timestamp":1787805577000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/jssc.70513"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8]]},"references-count":42,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2026,8]]}},"alternative-id":["10.1002\/jssc.70513"],"URL":"https:\/\/doi.org\/10.1002\/jssc.70513","archive":["Portico"],"relation":{},"ISSN":["1615-9306","1615-9314"],"issn-type":[{"value":"1615-9306","type":"print"},{"value":"1615-9314","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8]]},"assertion":[{"value":"2026-04-25","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-16","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-25","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70513"}}