{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T20:53:47Z","timestamp":1792270427529,"version":"4.4.0"},"reference-count":52,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2026,9,13]],"date-time":"2026-09-13T00:00:00Z","timestamp":1789257600000},"content-version":"vor","delay-in-days":12,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The robustness of a test for characterizing hydrophilic interaction liquid chromatography (HILIC) columns against variations in aqueous mobile\u2010phase composition was evaluated using mobile phases of acetonitrile\/aqueous mixture (9:1, v\/v) in the pH range of 3.6\u20136.8 of the aqueous part. Ten columns (commercially available and home\u2010made columns) were examined under five mobile phases differing in anion species (formate or acetate), NH\n                    <jats:sub>4<\/jats:sub>\n                    <jats:sup>+<\/jats:sup>\n                    concentration and salt concentration. The specific hydrophilicity of the columns was assessed via the retention factor of uridine (\n                    <jats:italic>k<\/jats:italic>\n                    (U)), and the relative retention characteristics (i.e., retention selectivity:\n                    <jats:italic>\u03b1<\/jats:italic>\n                    ) were evaluated using structure\u2010related probe pairs. The\n                    <jats:italic>k<\/jats:italic>\n                    (U)\u2010values exhibited low variability under low\u2010salt conditions (20\u00a0mM in the aqueous part; CVs = 0.01\u20130.08), but higher variability under high\u2010salt conditions (100\u00a0mM in the aqueous part; CVs = 0.03\u20130.18). Under comparable pH conditions (ammonium formate at pH 4.5\u00a0vs. ammonium acetate at pH 4.7),\n                    <jats:italic>k<\/jats:italic>\n                    (U)\u2010values were consistently higher in the former, indicating that differences in NH\n                    <jats:sub>4<\/jats:sub>\n                    <jats:sup>+<\/jats:sup>\n                    concentration affect hydration at the stationary\u2010phase interface and thereby influence retention. This suggests that\n                    <jats:italic>k<\/jats:italic>\n                    (U), commonly used as an indicator of hydrophilic retention, can be affected significantly by partition interactions that are controlled by ionic concentration rather than the pH value of the mobile phase. In contrast, the characteristic\n                    <jats:italic>\u03b1<\/jats:italic>\n                    \u2010values primarily associated with solute retention via partitioning exhibited high robustness (CVs \u2264 0.03). Notably,\n                    <jats:italic>\u03b1<\/jats:italic>\n                    (\n                    <jats:italic>k<\/jats:italic>\n                    (U)\/\n                    <jats:italic>k<\/jats:italic>\n                    (2\u2032\u2010deoxyuridine)) values correlated with the estimated phase ratio, suggesting that it reflects the effective volume of the hydrated layer on the surface of the packing materials. Furthermore, the relationships between\n                    <jats:italic>\u03b1<\/jats:italic>\n                    (\n                    <jats:italic>k<\/jats:italic>\n                    (1\u2010\u03b2\u2010D\u2010arabinofuranosyl uracil)\/\n                    <jats:italic>k<\/jats:italic>\n                    (U)) and\n                    <jats:italic>\u03b1<\/jats:italic>\n                    (\n                    <jats:italic>k<\/jats:italic>\n                    (vidarabine)\/\n                    <jats:italic>k<\/jats:italic>\n                    (adenosine)) indicated that nucleobase\u2011dependent interactions contribute significantly to stationary\u2011phase retention. Overall, these results demonstrate that each\n                    <jats:italic>k<\/jats:italic>\n                    (U) and\n                    <jats:italic>\u03b1<\/jats:italic>\n                    , particularly those associated with partition\u2010dominated retention, exhibit generally high robustness within this test framework and provide a basis for more robust and transferable HILIC column characterization.\n                  <\/jats:p>","DOI":"10.1002\/jssc.70520","type":"journal-article","created":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T05:47:47Z","timestamp":1789364867000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Systematic Evaluation of Partition\u2010Dominated Retention in Hydrophilic Interaction Liquid Chromatography (HILIC) Under Varying Aqueous Components of the Mobile Phase: Revisiting the HILIC Column Test Scheme"],"prefix":"10.1002","volume":"49","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-3046-4389","authenticated-orcid":false,"given":"Tomoaki","family":"Shimpo","sequence":"first","affiliation":[{"name":"Faculty of Molecular Chemistry and Engineering Kyoto Institute of Technology  Kyoto Japan"},{"name":"LC Business Unit of Life Science Business Department Analytical &amp; Measuring Instruments Division Shimadzu Corporation  Kyoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7365-7271","authenticated-orcid":false,"given":"Tohru","family":"Ikegami","sequence":"additional","affiliation":[{"name":"Faculty of Molecular Chemistry and Engineering Kyoto Institute of Technology  Kyoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,13]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021\u20109673(00)96972\u20103"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.200600199"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1134\/S1061934819050058"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aca.2011.02.047"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00216\u2010011\u20105308\u20105"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2025.466520"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2024.465524"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules30173567"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2024.465467"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1021\/jp3083562"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1021\/jp312501b"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2013.09.061"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2017.06.026"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.3390\/analytica6040039"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.3390\/separations6020019"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules28186459"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.202200165"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2018.01.038"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2011.06.037"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2011.06.048"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2020.461850"},{"key":"e_1_2_9_23_1","first-page":"102","article-title":"Retention and Selectivity of Stationary Phases Used in HILIC","volume":"28","author":"Bell D. 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