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To address this issue, a metabolism\u2010oriented strategy was established and applied to Wuao Sanzi Decoction (WASZD), a classic TCM formula for respiratory diseases such as asthma. Ultra\u2010high\u2010performance liquid chromatography coupled with quadrupole time\u2010of\u2010flight tandem mass spectrometry was used for systematic analysis of the chemicalome, in vivo prototypes, metabolites, and tissue distribution of WASZD. A total of 200 chemical components were identified in vitro, including 102 flavonoids, 29 triterpenoids, 10 steroids, 14 organic acids, and 45 other compounds. Further, 91 prototypes and 212 metabolites in vivo were characterized in biological samples of rats. Key metabolic pathways involved phase I reactions (reduction, oxidation, and demethylation), phase II reactions (glucuronidation, methylation, and sulfation), and gut microbiota\u2010mediated ring cleavage of flavonoids, which generated bioactive small\u2010molecule phenolic acids. Tissue distribution analysis revealed that prototypes and metabolites were predominantly enriched in the ileum, colon, liver, kidney, and lung, with flavonoids and their metabolites identified as the most abundant components, suggesting they are the core bioactive candidates of WASZD. This study not only putatively clarifies the pharmacodynamic material basis of WASZD but also establishes a reproducible technical framework for decoding the complex in vivo behavior of multi\u2010component TCM formulas, facilitating their modernization and rational clinical application.<\/jats:p>","DOI":"10.1002\/jssc.70521","type":"journal-article","created":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T14:42:59Z","timestamp":1788273779000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A Metabolism\u2010Oriented Strategy for Systematic Analysis of the Pharmacological Material Basis of Classic Traditional Chinese Medicine Formulas: Taking Wuao Sanzi Decoction as an Example"],"prefix":"10.1002","volume":"49","author":[{"given":"Jin\u2010Jin","family":"Liao","sequence":"first","affiliation":[{"name":"Pukou Hospital of Chinese Medicine Affiliated to China Pharmaceutical University Department of Chinese 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