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Here, an integrated analytical workflow combining reversed\u2010phase liquid chromatography (RPLC) and hydrophilic interaction liquid chromatography (HILIC) with quadrupole time\u2010of\u2010flight mass spectrometry (Q\u2010TOF\u2010MS), cellular assays, metabolomics, and network pharmacology was applied in an amyloid\u2010\u03b2\n                    <jats:sub>1\u201042<\/jats:sub>\n                    (A\u03b2\n                    <jats:sub>1\u201042<\/jats:sub>\n                    ) oligomer\u2010induced human brain microvascular endothelial cell (hBMEC) injury model. The complementary RPLC\/HILIC and positive\/negative electrospray\u2010ionization strategy expanded coverage across a broad polarity range. UHPLC\u2010Q\/TOF\u2010MS detected 90 compounds, mainly phenolic acids, phthalides, tanshinones, diterpenoids, and other aromatic or terpenoid constituents. The reductions in cell viability and zonula occludens\u20101 expression following A\u03b2\n                    <jats:sub>1\u201042<\/jats:sub>\n                    exposure confirmed the successful establishment of the cellular injury model, whereas pretreatment with Danshen, Chuanxiong, or their combination increased cell viability and alleviated A\u03b2\n                    <jats:sub>1\u201042<\/jats:sub>\n                    \u2010induced cellular injury. Untargeted metabolomics annotated 38 differential metabolites, of which 30 showed a recovery\u2010associated shift toward the control state after Danshen\u2013Chuanxiong treatment; the associated pathways included amino\u2010acid, purine, glutathione, and glycerophospholipid metabolism. Integrative analysis prioritized seven candidate targets of Danshen\u2013Chuanxiong, namely ACHE, VEGFA, NOS2, NOS3, AKR1B1, MDM2, and XDH. These findings provide a separation\u2010MS\u2010based chemical characterization and mechanistic framework for further validation of Danshen\u2013Chuanxiong in AD\u2010related endothelial injury models.\n                  <\/jats:p>","DOI":"10.1002\/jssc.70527","type":"journal-article","created":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T07:46:16Z","timestamp":1788939976000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["UHPLC\u2010Q\/TOF\u2010MS Characterization of Danshen\u2013Chuanxiong and Mechanistic Insights Into Its Protective Effects Against A\u03b2\n                    <sub>1\u201042<\/sub>\n                    \u2010Induced Brain Endothelial Injury Through Metabolomics"],"prefix":"10.1002","volume":"49","author":[{"given":"Hui","family":"Wang","sequence":"first","affiliation":[{"name":"School of Pharmacy Naval Medical University  Shanghai 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