{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T10:56:06Z","timestamp":1790592966711,"version":"4.1.0"},"reference-count":15,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T00:00:00Z","timestamp":1788998400000},"content-version":"vor","delay-in-days":9,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100004735","name":"Natural Science Foundation of Hunan Province","doi-asserted-by":"publisher","award":["2024JJ5252"],"award-info":[{"award-number":["2024JJ5252"]}],"id":[{"id":"10.13039\/501100004735","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100014472","name":"Scientific Research Foundation of Hunan Provincial Education Department","doi-asserted-by":"publisher","award":["23B0071"],"award-info":[{"award-number":["23B0071"]}],"id":[{"id":"10.13039\/100014472","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2026,9]]},"abstract":"ABSTRACT<\/jats:title>\n \n The development of green and efficient methods for genomic DNA extraction from animal tissues is crucial for molecular diagnostics, food traceability, and genetic research. Conventional methods often involve toxic reagents, multiple centrifugation steps, and are time\u2010consuming. In this study, a hydrophobic magnetic ionic liquid (MIL),\n N<\/jats:italic>\n \u2010octyl\u20104\u2010dimethylaminopyridinium hexafluorophosphate MIL ([C\n 8<\/jats:sub>\n DMAP][PF\n 6<\/jats:sub>\n ]\u2011Ni MIL), was synthesized and applied for the selective extraction of genomic DNA from various animal tissues. The material exhibited strong paramagnetic behavior, high thermal stability, and excellent hydrophobicity, enabling rapid phase separation under an external magnetic field. A mechanical shaking\u2010assisted extraction method was developed, and key parameters\u00a0including temperature, time, shaking speed, and [C\n 8<\/jats:sub>\n DMAP][PF\n 6<\/jats:sub>\n ]\u2010Ni MIL dosage were systematically optimized. The method demonstrated high selectivity for DNA over proteins, RNA, and amino acids, with a maximum recovery rate of 78.06 \u00b1 1.91%. Compared to a commercial DNA extraction kit, the [C\n 8<\/jats:sub>\n DMAP][PF\n 6<\/jats:sub>\n ]\u2010Ni MIL\u2010based approach provided higher yields from several tissues, including mouse liver, brain, and rabbit lung. Furthermore, the [C\n 8<\/jats:sub>\n DMAP][PF\n 6<\/jats:sub>\n ]\u2010Ni MIL could be reused for at least six cycles while maintaining extraction efficiency. This work not only provides a high\u2010performance material for DNA extraction, but also demonstrates a sustainable and easily retrievable liquid\u2010phase separation strategy, offering a generalizable platform for complex sample pretreatment.\n <\/jats:p>","DOI":"10.1002\/jssc.70533","type":"journal-article","created":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T05:24:52Z","timestamp":1789104292000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Selective Extraction of Genomic DNA From Animal Tissues Using a Hydrophobic Magnetic Ionic Liquid"],"prefix":"10.1002","volume":"49","author":[{"given":"Yu","family":"Hou","sequence":"first","affiliation":[{"name":"School of Life Sciences Shanghai University Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xingchen","family":"He","sequence":"additional","affiliation":[{"name":"School of Life Sciences Hunan Normal University Changsha China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Tang","sequence":"additional","affiliation":[{"name":"School of Life Sciences Hunan Normal University Changsha China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7962-5510","authenticated-orcid":false,"given":"Xueqin","family":"Ding","sequence":"additional","affiliation":[{"name":"School of Life Sciences Shanghai University Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,10]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.2144\/000113672"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/jmammal\/gyae044"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.margen.2017.11.003"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/25.22.4692"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.3389\/fgene.2020.00374"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s002390010156"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/1755\u20100998.12616"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1099\/00221287\u2010132\u20102\u2010541"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2018.07.019"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aca.2016.07.022"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1021\/cr980032t"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1246\/cl.2004.1590"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.trac.2019.02.007"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1039\/C4RA05156D"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.langmuir.1c01567"}],"container-title":["Journal of Separation Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jssc.70533","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jssc.70533","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jssc.70533","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T10:01:50Z","timestamp":1790589710000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/jssc.70533"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9]]},"references-count":15,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2026,9]]}},"alternative-id":["10.1002\/jssc.70533"],"URL":"https:\/\/doi.org\/10.1002\/jssc.70533","archive":["Portico"],"relation":{},"ISSN":["1615-9306","1615-9314"],"issn-type":[{"value":"1615-9306","type":"print"},{"value":"1615-9314","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9]]},"assertion":[{"value":"2026-01-29","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-04","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-10","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70533"}}