{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T10:56:06Z","timestamp":1790592966227,"version":"4.1.0"},"reference-count":35,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T00:00:00Z","timestamp":1789603200000},"content-version":"vor","delay-in-days":16,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    A sensitive three\u2010step online enrichment strategy including large\u2010volume sample stacking, dynamic pH junction, and sweeping was successfully developed and validated for the simultaneous online enrichment and separation of phthalides and phenolic acids in Chuanxiong Rhizoma (CX) by coupling reversed migration cyclodextrin\u2010modified micellar electrokinetic chromatography (RM\u2010CD\u2010MEKC) with a diode array detector. The samples were prepared in a low\u2010conductivity, high\u2010pH matrix consisting of 3\u00a0mM borax buffer at pH 7.5. The background electrolyte was a high\u2010conductivity, low\u2010pH buffer containing 10 mg\u00b7mL\n                    <jats:sup>\u22121<\/jats:sup>\n                    sulfobutylether\u2010\u03b2\u2010cyclodextrin, 80\u00a0mM sodium dodecyl sulfate, and 25\u00a0mM sodium dihydrogen phosphate in 20% methanol (pH 3.5). Under the optimized conditions, four major active components (senkyunolide A, senkyunolide I, ferulic acid, and chlorogenic acid) of\n                    <jats:italic>Ligusticum chuanxiong<\/jats:italic>\n                    Hort. were efficiently separated within 17\u00a0min, achieving good resolution and 10\u2010 to 44\u2010fold enrichment. The limits of detection ranged from 0.09 to 0.25 \u00b5g\u00b7mL\n                    <jats:sup>\u22121<\/jats:sup>\n                    while the limits of quantification ranged from 0.31 to 2.24 \u00b5g\u00b7mL\n                    <jats:sup>\u22121<\/jats:sup>\n                    . The relative standard deviations for intra\u2010day and inter\u2010day precision were all below 3.45%, demonstrating good precision and method stability. Overall, the proposed RM\u2010CD\u2010MEKC combined with a three\u2010step enrichment technique provides a simple, rapid, and environmentally friendly enrichment approach for the simultaneous determination of phthalides and phenolic acids in CX samples.\n                  <\/jats:p>","DOI":"10.1002\/jssc.70534","type":"journal-article","created":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T13:09:16Z","timestamp":1789650556000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Online Enrichment and Separation of Phthalides and Phenolic Acids From Chuanxiong Rhizoma by Reversed Migration Cyclodextrin\u2010Modified Micellar Electrokinetic Chromatography Combined With a Three\u2010Step Enrichment Technique"],"prefix":"10.1002","volume":"49","author":[{"given":"Liping","family":"Huang","sequence":"first","affiliation":[{"name":"State Key Laboratory of Chinese Medicine Modernization Tianjin University of Traditional 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