{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T10:56:07Z","timestamp":1790592967092,"version":"4.1.0"},"reference-count":40,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T00:00:00Z","timestamp":1789171200000},"content-version":"vor","delay-in-days":11,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["22574129"],"award-info":[{"award-number":["22574129"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100017596","name":"Natural Science Basic Research Program of Shaanxi Province","doi-asserted-by":"publisher","award":["2024JC\u2010TBZC\u201021"],"award-info":[{"award-number":["2024JC\u2010TBZC\u201021"]}],"id":[{"id":"10.13039\/501100017596","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Separation Science"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Current mainstream treatment of chronic obstructive pulmonary disease (COPD) relies largely on long\u2010acting \u03b2\n                    <jats:sub>2<\/jats:sub>\n                    \u2010adrenergic receptor (\u03b2\n                    <jats:sub>2<\/jats:sub>\n                    AR) agonists and muscarinic M\n                    <jats:sub>3<\/jats:sub>\n                    receptor (M\n                    <jats:sub>3<\/jats:sub>\n                    R) antagonists, yet residual inflammation and variable treatment responses persist. Developing therapeutics from multicomponent herbal medicines is an effective drug development approach but is constrained by the difficulty of linking individual constituents to pharmacologically relevant targets and in vivo efficacy. This study established an integrated discovery workflow combining whole\u2010formula efficacy testing, receptor\u2010affinity chromatography, mass spectrometry (MS), and in vivo validation of candidate combinations. We applied this workflow to the Shaoqitongfei formula (SQTF), an investigator\u2010developed three\u2010herb prescription evaluated for ameliorating COPD, and selected the \u03b2\n                    <jats:sub>2<\/jats:sub>\n                    AR and M\n                    <jats:sub>3<\/jats:sub>\n                    R as clinically relevant bronchodilator targets. In porcine pancreatic elastase\/lipopolysaccharide (LPS)\u2010induced COPD mice, SQTF improved respiratory parameters, attenuated lung injury, and reduced inflammatory cytokine levels in serum and bronchoalveolar lavage fluid. Affinity columns containing immobilized \u03b2\n                    <jats:sub>2<\/jats:sub>\n                    AR and M\n                    <jats:sub>3<\/jats:sub>\n                    R stationary phases were utilized to screen the receptor\u2010targeting components in formula extract. High\u2010performance liquid chromatography\u2010tandem MS analysis tentatively annotated albiflorin and kaempferol in the extract retained by both columns. In rats exposed to cigarette smoke and LPS, co\u2010administration of albiflorin and kaempferol reproduced several of the pulmonary and anti\u2010inflammatory effects of SQTF and shifted protein\u2010expression patterns associated with signaling proteins to those observed in controls. Taken together, these findings establish a receptor\u2010guided workflow for selecting pharmacologically relevant constituent combinations from complex herbal medicines and provide an analytical basis for the further development of SQTF\u2010derived therapies for COPD.\n                  <\/jats:p>","DOI":"10.1002\/jssc.70535","type":"journal-article","created":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T08:11:58Z","timestamp":1789200718000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Selective Screening of Dual\u2010Target Bioactive Constituents From Shaoqitongfei Formula Against Chronic Obstructive Pulmonary Disease by Receptor Chromatography"],"prefix":"10.1002","volume":"49","author":[{"given":"Yunshan","family":"Wang","sequence":"first","affiliation":[{"name":"Key Laboratory of Resource Biology and Biotechnology in Western China Ministry of Education College of Life Sciences Northwest University  Xi'an China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yangyang","family":"Zhang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Resource Biology and Biotechnology in Western China Ministry of Education College of Life Sciences Northwest University  Xi'an China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kexin","family":"Li","sequence":"additional","affiliation":[{"name":"Key Laboratory of Resource Biology and Biotechnology in Western China Ministry of Education College of Life Sciences Northwest University  Xi'an China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zihu","family":"Wang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Resource Biology and Biotechnology in Western China Ministry of Education College of Life Sciences Northwest University  Xi'an China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jia","family":"Wang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Resource Biology and Biotechnology in Western China Ministry of Education College of Life Sciences Northwest University  Xi'an China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qian","family":"Li","sequence":"additional","affiliation":[{"name":"Key Laboratory of Resource Biology and Biotechnology in Western China Ministry of Education College of Life Sciences Northwest University  Xi'an China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,12]]},"reference":[{"key":"e_1_2_9_2_1","volume-title":"Global Strategy for Prevention, Diagnosis and Management of COPD: 2026 Report","author":"Global Initiative for Chronic Obstructive Lung Disease","year":"2026"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1001\/jamanetworkopen.2023.46598"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2213\u20102600(21)00511\u20107"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra1900500"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1183\/13993003.00239\u20102023"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.202003\u20100625ST"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1513\/pats.200504\u2010027SR"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1186\/1465\u20109921\u20104\u20102"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010024\u201051991\u20106"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s41664\u2010024\u201000311\u20104"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s41664\u2010025\u201000395\u20106"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/s41664\u2010025\u201000393\u20108"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/s41664\u2010024\u201000324\u2010z"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s41664\u2010024\u201000305\u20102"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.202000708"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.202300129"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.70358"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/s41664\u2010024\u201000315\u20100"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cclet.2024.109792"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2021.462443"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10753\u2010018\u20100811\u2010x"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2018.01001"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu12040926"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12890\u2010024\u201003365\u20103"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2022.830859"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1165\/ajrcmb.31.3.1"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.3390\/toxics9040072"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1172\/jci167337"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1093\/function\/zqac050"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.coph.2014.03.005"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/bmc.3292"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jasms.2006.12.011"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules29225246"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1039\/c5ay02809d"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phymed.2023.154774"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010021\u201026777\u20109"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010022\u201035253\u2010x"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2214024119"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1165\/rcmb.2021\u20100345OC"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phymed.2023.154980"}],"container-title":["Journal of Separation Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jssc.70535","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jssc.70535","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jssc.70535","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T10:01:56Z","timestamp":1790589716000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/jssc.70535"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9]]},"references-count":40,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2026,9]]}},"alternative-id":["10.1002\/jssc.70535"],"URL":"https:\/\/doi.org\/10.1002\/jssc.70535","archive":["Portico"],"relation":{},"ISSN":["1615-9306","1615-9314"],"issn-type":[{"value":"1615-9306","type":"print"},{"value":"1615-9314","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9]]},"assertion":[{"value":"2026-07-20","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-09","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-12","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70535"}}