{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,27]],"date-time":"2026-03-27T01:49:29Z","timestamp":1774576169174,"version":"3.50.1"},"reference-count":38,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2018,11,27]],"date-time":"2018-11-27T00:00:00Z","timestamp":1543276800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["Grant No. 81373699"],"award-info":[{"award-number":["Grant No. 81373699"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Ultrasound Medicine"],"published-print":{"date-parts":[[2019,7]]},"abstract":"<jats:sec><jats:title>Objectives<\/jats:title><jats:p>The aim of this study was to investigate whether the measurement of brain tissue stiffness using acoustic radiation force impulse (ARFI) elastography with virtual touch tissue quantification can improve the early detection of neonatal hypoxic\u2010ischemic brain damage in rats.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Seven\u2010day\u2010old Sprague\u2010Dawley rats were randomly assigned to 3 groups: the mild asphyxia (n\u2009=\u200930), moderate asphyxia (n\u2009=\u200930), and sham control (n\u2009=\u200910) groups. Rats in the mild and moderate asphyxia groups were exposed to 8% oxygen (hypoxia) for 30 and 60\u2009minutes, respectively, at 1\u2009hour after ligation of the right common carotid artery. An ultrasound diagnostic instrument was used to obtain 2\u2010dimensional ultrasound images, and ARFI with virtual touch tissue quantification was used to measure shear wave velocity preoperatively and at 12, 24, 48, and 72\u2009hours postoperatively. Hematoxylin\u2010eosin staining was used to evaluate brain damage.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Two\u2010dimensional ultrasound imaging detected swelling and increased echogenicity at 48 to 72\u2009hours in the mild asphyxia group and at 24 to 72\u2009hours in the moderate asphyxia group. The shear wave velocity substantially increased from 0.65\u2009\u00b1\u20090.04\u2009m\/s preoperatively to 0.78\u2009\u00b1\u20090.07\u2009m\/s at 72\u2009hours in the moderate asphyxia group and from 0.64\u2009\u00b1\u20090.04\u2009m\/s preoperatively to 0.70\u2009\u00b1\u20090.03\u2009m\/s at 72\u2009hours in the mild asphyxia group. The changes in the shear wave velocity coincided with the histopathologic changes in the brain, which included neuronal demyelination, hyperplasia, and necrosis; edema around vascular structures; and hemorrhage in the ependymal and periventricular areas.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Shear wave velocity data obtained with the virtual touch tissue quantification technique may be used for early diagnosis of neonatal hypoxic\u2010ischemic brain damage.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jum.14869","type":"journal-article","created":{"date-parts":[[2018,11,27]],"date-time":"2018-11-27T15:44:52Z","timestamp":1543333492000},"page":"1797-1805","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Acoustic Radiation Force Impulse Imaging With Virtual Touch Tissue Quantification Enables Characterization of Mild Hypoxic\u2010Ischemic Brain Damage in Neonatal Rats"],"prefix":"10.1002","volume":"38","author":[{"given":"Zhen\u2010Hua","family":"Zhu","sequence":"first","affiliation":[{"name":"Department of Otorhinolaryngology\u2010Head and Neck Surgery, The First Hospital Hunan University of Chinese Medical  Hunan Province China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ke\u2010ping","family":"Peng","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology\u2010Head and Neck Surgery, The First Hospital Hunan University of Chinese Medical  Hunan Province China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ming\u2010hui","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Ultrasound, The Second Xiangya Hospital Central South University  Hunan Province China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6282-269X","authenticated-orcid":false,"given":"Gui\u2010xiang","family":"Tian","sequence":"additional","affiliation":[{"name":"Department of Ultrasound, The Second Xiangya Hospital Central South University  Hunan Province China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,11,27]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00431-007-0437-8"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajog.2008.06.094"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.3109\/14767058.2011.587061"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1651-2227.1995.tb13794.x"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1001\/archpediatrics.2011.1772"},{"issue":"4","key":"e_1_2_6_7_1","first-page":"CD003311","article-title":"Cooling for newborns with hypoxic ischaemic encephalopathy","author":"Jacobs SE","year":"2003","journal-title":"Cochrane Database Syst Rev"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.nainr.2011.07.004"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12028-009-9324-7"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1542\/neo.15-5-e177"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/ped.12233"},{"key":"e_1_2_6_12_1","unstructured":"GuideBI. 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