{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,22]],"date-time":"2026-01-22T15:14:59Z","timestamp":1769094899903,"version":"3.49.0"},"reference-count":23,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2024,3,5]],"date-time":"2024-03-05T00:00:00Z","timestamp":1709596800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Ultrasound Medicine"],"published-print":{"date-parts":[[2024,6]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Acoustically activatable perfluoropropane droplets (PD) can be formulated from commercially available microbubble preparations. Diagnostic transthoracic ultrasound frequencies have resulted in acoustic activation (AA) predominately within myocardial infarct zones (IZ).<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>We hypothesized that the AA area following acute coronary ischemia\/reperfusion (I\/R) would selectively enhance the developing scar zone, and target bioeffects specifically to this region.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We administered intravenous PD in 36 rats and 20 pigs at various stages of myocardial scar formation (30\u2009minutes, 1\u2009day, and 7\u2009days post I\/R) to determine what effect infarct age had on the AA within the IZ. This was correlated with histology, myeloperoxidase activity, and tissue nitrite activity.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The degree of AA within the IZ in rats was not associated with collagen content, neutrophil infiltration, or infarct age. AA within 24\u2009hours of I\/R was associated with increased nitric oxide utilization selectively within the IZ (<jats:italic>P<\/jats:italic>\u2009&lt;\u2009.05 compared with remote zone). The spatial extent of AA in pigs correlated with infarct size only when performed before sacrifice at 7\u2009days (<jats:italic>r<\/jats:italic>\u2009=\u2009.74, <jats:italic>P<\/jats:italic>\u2009&lt;\u2009.01).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Acoustic activation of intravenous PD enhances the developing scar zone following I\/R, and results in selective tissue nitric oxide utilization.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jum.16435","type":"journal-article","created":{"date-parts":[[2024,3,5]],"date-time":"2024-03-05T09:48:53Z","timestamp":1709632133000},"page":"1063-1080","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Acoustic Activation Imaging With Intravenous Perfluoropropane Nanodroplets Results in Selective Bioactivation of the Risk Area"],"prefix":"10.1002","volume":"43","author":[{"given":"Shouqiang","family":"Li","sequence":"first","affiliation":[{"name":"Department of Ultrasound Second Affiliated Hospital of Harbin Medical University  Harbin China"},{"name":"Division of Cardiovascular Medicine University of Nebraska Medical Center  Omaha Nebraska USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cheng","family":"Chen","sequence":"additional","affiliation":[{"name":"Center for Ultrasound Molecular Imaging and Therapeutics University of Pittsburgh  Pittsburgh Pennsylvania USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John","family":"Lof","sequence":"additional","affiliation":[{"name":"Division of Cardiovascular Medicine University of Nebraska Medical Center  Omaha Nebraska USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elizabeth A.","family":"Stolze","sequence":"additional","affiliation":[{"name":"Division of Cardiovascular Medicine University of Nebraska Medical Center  Omaha Nebraska USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiri","family":"Sklenar","sequence":"additional","affiliation":[{"name":"narnar.co  Lake Oswego Oregon USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xucai","family":"Chen","sequence":"additional","affiliation":[{"name":"Center for Ultrasound Molecular Imaging and Therapeutics University of Pittsburgh  Pittsburgh Pennsylvania USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John J.","family":"Pacella","sequence":"additional","affiliation":[{"name":"Center for Ultrasound Molecular Imaging and Therapeutics University of Pittsburgh  Pittsburgh Pennsylvania USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Flordeliza S.","family":"Villanueva","sequence":"additional","affiliation":[{"name":"Center for Ultrasound Molecular Imaging and Therapeutics University of Pittsburgh  Pittsburgh Pennsylvania USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Terry O.","family":"Matsunaga","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering University of Arizona  Tucson Arizona USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E. Carr","family":"Everbach","sequence":"additional","affiliation":[{"name":"Department of Engineering Swarthmore College  Swarthmore Pennsylvania USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stanley J.","family":"Radio","sequence":"additional","affiliation":[{"name":"Department of Pathology and Microbiology University of Nebraska Medical Center  Omaha Nebraska USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sherry N.","family":"Westphal","sequence":"additional","affiliation":[{"name":"Division of Cardiovascular Medicine University of Nebraska Medical Center  Omaha Nebraska USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sruti","family":"Shiva","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Chemical Biology, Molecular Medicine Institute University of Pittsburgh  Pittsburgh Pennsylvania 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