{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T16:48:28Z","timestamp":1785775708355,"version":"3.56.0"},"reference-count":22,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2025,4,8]],"date-time":"2025-04-08T00:00:00Z","timestamp":1744070400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2020YFA0908800"],"award-info":[{"award-number":["2020YFA0908800"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005046","name":"Natural Science Foundation of Heilongjiang Province","doi-asserted-by":"publisher","award":["PL2024H156"],"award-info":[{"award-number":["PL2024H156"]}],"id":[{"id":"10.13039\/501100005046","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Ultrasound Medicine"],"published-print":{"date-parts":[[2025,8]]},"abstract":"<jats:sec><jats:title>Objectives<\/jats:title><jats:p>Super\u2010resolution ultrasound microvascular imaging (SRUS) has emerged as a noninvasive technology capable of visualizing the microvasculature with exceptional spatial resolution, surpassing the acoustic diffraction limit. This study aims to assess the potential of SRUS in staging liver fibrosis by evaluating its diagnostic performance against ultrasound viscosity imaging.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Liver fibrosis was induced by carbon tetrachloride (CCl<jats:sub>4<\/jats:sub>) in 30 mice. The mice were evenly distributed across five stages (6 mice per stage), categorized from F0 (no fibrosis) to F4 (cirrhosis) based on the extent of collagen deposition. SRUS microvascular imaging and ultrasound viscosity imaging were compared for their efficacy in detecting liver fibrosis stages. Immunohistochemistry and histopathological analyses were conducted to correlate vessel density and collagen deposition.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>SRUS effectively detected microvascular changes across all fibrosis stages. Significant vessel diameter enlargement was observed at early stages (F1), with further increases in advanced stages (F3\u2013F4). Vessel density significantly decreased in later stages, indicating compromised angiogenesis. Ultrasound viscosity imaging showed marked viscoelastic reductions in fibrosis but lacked sensitivity in early\u2010stage detection. SRUS parameters exhibited strong correlations with histological findings, underscoring their potential diagnostic value. Receiver operating characteristic (ROC) curve analysis further demonstrated the superior sensitivity of SRUS (89.59% [95% confidence interval (CI): 84.87\u201392.96%]), particularly in distinguishing early\u2010stage fibrosis (F0\u2013F1) from advanced stages (F2\u2013F4) (area under the curve [AUC]\u2009=\u20090.9610, 95% CI: 0.9449\u20130.9771; <jats:italic>P<\/jats:italic>\u2009&lt;\u2009.001).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>SRUS microvascular imaging is a promising adjunct to traditional elastography, offering enhanced sensitivity for early\u2010stage liver fibrosis detection. It provides critical insights into microcirculatory dysfunction, complementing stiffness measurements and aiding in accurate diagnosis.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jum.16703","type":"journal-article","created":{"date-parts":[[2025,4,8]],"date-time":"2025-04-08T09:35:37Z","timestamp":1744104937000},"page":"1481-1491","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Proof of Concept: Super\u2010Resolution Ultrasound and Viscoelastic Imaging of Hepatic Microcirculation for Early Detection and Staging of Liver Fibrosis in a Murine Model"],"prefix":"10.1002","volume":"44","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-7521-9785","authenticated-orcid":false,"given":"Rui","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Cardiovascular Ultrasound the Fourth Affiliated Hospital of Harbin Medical University  Harbin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jieqiong","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Rehabilitation Medicine, Huashan Hospital Fudan University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tingting","family":"Liu","sequence":"additional","affiliation":[{"name":"Ultrasonic Medicine, Graduate School Guangxi University of Chinese Medicine  Nanning China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Cardiovascular Ultrasound the Fourth Affiliated Hospital of Harbin Medical University  Harbin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuai","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Clinical and Research Shenzhen Mindray Bio\u2010Medical Electronics Co., Ltd.  Shenzhen China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fei","family":"Yan","sequence":"additional","affiliation":[{"name":"Center for Cell and Gene Circuit Design, CAS Key Laboratory of Quantitative Engineering Biology, Shenzhen Institute of Synthetic Biology, Shenzhen Institutes of Advanced Technology Chinese Academy of Sciences  Shenzhen China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Xue","sequence":"additional","affiliation":[{"name":"Department of Cardiovascular Ultrasound the Fourth Affiliated Hospital of Harbin Medical University  Harbin China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,4,8]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41575-020-00372-7"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(21)01374-X"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2023.05.053"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mam.2023.101231"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.addr.2021.113888"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.3350\/cmh.2020.0181"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1148\/radiol.212609"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2024.04.031"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2021.06.002"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41575-018-0097-3"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhepr.2021.100316"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.compbiomed.2023.107563"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/apt.14279"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1117\/1.JBO.19.11.116006"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature16066"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41551-021-00697-x"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1148\/radiol.2015141941"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41551-024-01206-6"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.14366\/usg.21136"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1148\/radiol.212325"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.22007"},{"key":"e_1_2_8_23_1","first-page":"1","article-title":"Alterations to hepatic microcirculation in thioacetam\u2010ide\u2010induced cirrhotic livers of rats","volume":"48","author":"Nakata M","year":"2002","journal-title":"Osaka City Med J"}],"container-title":["Journal of Ultrasound in Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jum.16703","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,7,14]],"date-time":"2025-07-14T14:22:47Z","timestamp":1752502967000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jum.16703"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,4,8]]},"references-count":22,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2025,8]]}},"alternative-id":["10.1002\/jum.16703"],"URL":"https:\/\/doi.org\/10.1002\/jum.16703","archive":["Portico"],"relation":{},"ISSN":["0278-4297","1550-9613"],"issn-type":[{"value":"0278-4297","type":"print"},{"value":"1550-9613","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,4,8]]},"assertion":[{"value":"2024-11-04","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-03-28","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-04-08","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}