{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T09:58:34Z","timestamp":1753869514184,"version":"3.41.2"},"reference-count":24,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2025,4,17]],"date-time":"2025-04-17T00:00:00Z","timestamp":1744848000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Ultrasound Medicine"],"published-print":{"date-parts":[[2025,8]]},"abstract":"<jats:sec><jats:title>Objective<\/jats:title><jats:p>It is difficult to quickly assess the degree of testicular injury in patients with blunt testicular trauma, and accurate and rapid assessment of the degree of injury is related to clinical decision\u2010making and treatment outcomes. This study explored the sonographic characteristics of patients with different degrees of blunt testicular trauma via contrast\u2010enhanced ultrasound (CEUS), as well as biomarkers of oxidative stress and vascular endothelial cell injury, to further evaluate the diagnostic value of these parameters in blunt testicular trauma.<\/jats:p><\/jats:sec><jats:sec><jats:title>Method<\/jats:title><jats:p>New Zealand male rabbits were used to establish blunt testicular trauma models of different degrees, including contusion, hematoma, rupture, and control models.<\/jats:p><jats:p>Routine ultrasound and CEUS were performed immediately, 2, 4, and 6\u2009h after modeling, and pathological examination was used to confirm the modeling results further. The levels of von Willebrand factor (vWF), endothelin\u20101 (ET\u20101), reactive oxygen species (ROS), and nitric oxide (NO) in the serum were analyzed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>According to the CEUS results, the arrival time (AT) (<jats:italic>F<\/jats:italic>\u2009=\u200935.159, <jats:italic>P<\/jats:italic>\u2009&lt;\u2009.001), time to peak intensity (TTP) (<jats:italic>F<\/jats:italic>\u2009=\u2009122.434; <jats:italic>P<\/jats:italic>\u2009&lt;\u2009.001), and area under the curve (AUC) (<jats:italic>F<\/jats:italic>\u2009=\u200947.444; <jats:italic>P<\/jats:italic>\u2009&lt;\u2009.001) of the time\u2010intensity curves showed significant differences among the groups. There was no significant difference between Hematoma Trauma (HT) (<jats:italic>F<\/jats:italic>\u2009=\u20090.178; <jats:italic>P<\/jats:italic>\u2009=\u2009.91) and Peak Intensity (PI) (<jats:italic>F<\/jats:italic>\u2009=\u20090.172; <jats:italic>P<\/jats:italic>\u2009=\u2009.92). For biomarkers, there were significant differences in vWF (<jats:italic>F<\/jats:italic>\u2009=\u200940.61; <jats:italic>P<\/jats:italic>\u2009&lt;\u2009.001), ROS (<jats:italic>F<\/jats:italic>\u2009=\u2009106.3; <jats:italic>P<\/jats:italic>\u2009&lt;\u2009.001), NO (<jats:italic>F<\/jats:italic>\u2009=\u200979.3; <jats:italic>P<\/jats:italic>\u2009&lt;\u2009.001), and ET\u20101 (<jats:italic>F<\/jats:italic>\u2009=\u200961.94; <jats:italic>P<\/jats:italic>\u2009&lt;\u2009.001) levels among the groups.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>For blunt testicular trauma, the AT, TTP, and AUC indices of CEUS can more accurately distinguish the degree of testicular injury. Oxidative stress and endothelial cell damage may also be used as biomarkers for evaluating the degree of testicular injury.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jum.16704","type":"journal-article","created":{"date-parts":[[2025,4,17]],"date-time":"2025-04-17T04:40:16Z","timestamp":1744864816000},"page":"1493-1502","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Changes in Contrast\u2010Enhanced Ultrasonography Results, Vascular Endothelial Cell Damage, and Oxidative Stress Indicators in Rabbit Models With Varying Degrees of Testicular Injury"],"prefix":"10.1002","volume":"44","author":[{"given":"Yaxi","family":"Zhao","sequence":"first","affiliation":[{"name":"Department of Ultrasound Affiliated Hospital of North Sichuan Medical College  Nanchong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haifei","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Vascular Surgery Affiliated Hospital of North Sichuan Medical College  Nanchong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gaofeng","family":"Su","sequence":"additional","affiliation":[{"name":"Department of Ultrasound Affiliated Hospital of North Sichuan Medical College  Nanchong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-2393-8207","authenticated-orcid":false,"given":"Yanbin","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Vascular Surgery Affiliated Hospital of North Sichuan Medical College  Nanchong 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