{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,8,15]],"date-time":"2025-08-15T00:47:18Z","timestamp":1755218838506,"version":"3.43.0"},"reference-count":25,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2025,5,5]],"date-time":"2025-05-05T00:00:00Z","timestamp":1746403200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82102054"],"award-info":[{"award-number":["82102054"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Ultrasound Medicine"],"published-print":{"date-parts":[[2025,9]]},"abstract":"<jats:sec><jats:title>Objectives<\/jats:title><jats:p>To assess the severity of intrauterine adhesion (IUA) after endometrial injury via ultrasound molecular imaging (USMI) of vascular endothelial growth factor receptor 2 (VEGFR2) expression on the uterine endothelium.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>MB<jats:sub>VEGFR2<\/jats:sub> was constructed via \u201cbiotin\u2010avidin\u2010biotin\u201d bridging technology. A rat IUA animal model was constructed via chemical injury. Fourteen days after the model was constructed, MB<jats:sub>Con<\/jats:sub> and MB<jats:sub>VEGFR2<\/jats:sub> contrast agents were injected into the standard group and the model group of rats, and simultaneous ultrasound molecular imaging was carried out and ultrasound molecular imaging was performed and the changes in ultrasound signals in vivo were normalized to differential targeted enhancement (dTE). In addition, immunohistochemistry was used to detect the expression levels of VEGFR2 and \u03b1\u2010SMA in the uterine tissues of the rats in both groups.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Targeted\u2010VEGFR2 microbubble (MB<jats:sub>VEGFR2<\/jats:sub>) was successfully constructed, the average particle size of the targeted VEGFR2 microbubble contrast agent was 2309.6\u2009\u00b1\u2009234.3\u2009nm, with the average zeta potential \u201016.9\u2009\u00b1\u20090.15\u2009mV and the dispersity coefficient less than 0.4. In addition, its essential performance and biosafety were examined, which revealed that the MB<jats:sub>VEGFR2<\/jats:sub> contrast agent had an appropriate size, stable imaging performance, good target adhesion, and good biocompatibility. In vivo, ultrasound imaging results revealed that after the injection of the targeted MB<jats:sub>VEGFR2<\/jats:sub>, the contrast ultrasound signal intensity of uterine tissues in the model group (62.5\u2009\u00b1\u200917.9\u2009dB) was higher than in the normal group (33.5\u2009\u00b1\u200912.5\u2009dB), and the difference was statistically significant (<jats:italic>P<\/jats:italic>\u2009&lt;\u2009.05). The immunohistochemistry results revealed that the expression of VEGFR2 and \u03b1\u2010SMA in the uterine tissue of the model group was greater than that in the standard group (<jats:italic>P\u2009&lt;<\/jats:italic>\u2009.05), further indicating that the signal intensity of the MB<jats:sub>VEGFR2<\/jats:sub> contrast agent can reflect the expression level of VEGFR2 molecules.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Ultrasound molecular imaging based on MB<jats:sub>VEGFR2<\/jats:sub> can be used to evaluate the expression level of VEGFR2 in uterine vascular endothelial cells. This provides a potential approach for the early diagnosis and treatment monitoring of intrauterine adhesions after endometrial injury.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jum.16710","type":"journal-article","created":{"date-parts":[[2025,5,5]],"date-time":"2025-05-05T09:41:28Z","timestamp":1746438088000},"page":"1555-1568","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Ultrasound Molecular Imaging With <scp>VEGFR2<\/scp>\u2010Targeted Microbubbles to Evaluate Intrauterine Adhesion After Endometrial Injury and Monitor the Therapeutic Effects"],"prefix":"10.1002","volume":"44","author":[{"given":"Likang","family":"Hou","sequence":"first","affiliation":[{"name":"Key Laboratory of Medical Imaging Precision Theranostics and Radiation Protection College of Hunan Province, the Affiliated Changsha Central Hospital, Hengyang Medical School, University of South China  Changsha China"},{"name":"Institute of Medical 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