{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T16:46:30Z","timestamp":1772988390187,"version":"3.50.1"},"reference-count":47,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2025,6,28]],"date-time":"2025-06-28T00:00:00Z","timestamp":1751068800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,6,28]],"date-time":"2025-06-28T00:00:00Z","timestamp":1751068800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Ultrasound Medicine"],"published-print":{"date-parts":[[2025,11]]},"abstract":"<jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>To establish a mouse model of trastuzumab\u2010induced cardiotoxicity that aligns with clinical standard treatment protocols, assess the extent of histological damage across various chambers of the mouse heart, and investigate whether 2\u2010dimensional speckle tracking echocardiography (2D\u2010STE) can detect early cardiac dysfunction, while comparing the diagnostic effectiveness of various parameters in cardiotoxicity.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Mice were randomly assigned to a trastuzumab group (Trz), a doxorubicin\u2010sequential\u2010trastuzumab group (Dox\u2010Trz), and a control group (Con). A mouse model of cardiotoxicity was developed according to clinical standard treatment protocols. Echocardiographic assessments were conducted prior to the experiment, at the end of the first, third, and fourth weeks, and at the conclusion of the experiment to measure and document cardiac function in the mice. Following this, the hearts of the mice were collected for histological analysis, including histopathological evaluation using H&amp;E staining, quantitative assessment of myocardial fibrosis using Masson staining, and evaluation of myocardial cell apoptosis using a TUNEL kit.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>H&amp;E staining revealed myocardial cell pathology in the hearts of mice in both the Trz and Dox\u2010Trz groups, whereas no abnormalities were observed in the Con group. Masson staining demonstrated a significant increase in interstitial fibrosis in the hearts of mice in the Trz and Dox\u2010Trz groups, with a higher proportion of collagen fibers in the atria compared to the ventricles, and a higher proportion in the right ventricle compared to the left ventricle, with statistical significance. TUNEL staining results indicated a statistically significant increase in myocardial cell apoptosis in the Trz and Dox\u2010Trz groups compared to the Con group. 2D\u2010STE showed significantly reduced left ventricular global longitudinal strain (GLS) and global radial strain (GRS) from baseline in both Trz and Dox\u2010Trz groups at T2. At T3, GLS and GRS were significantly lower in these groups compared to the Con group. Both groups also exhibited significantly reduced global circumferential strain (GCS) from baseline at T3, with the Dox\u2010Trz group showing significantly lower GCS than the Con group. All differences were statistically significant. However, left ventricular ejection fraction (LVEF), left ventricular fractional shortening (LVFS) parameter only exhibited intergroup differences at T4. Notably, GLS demonstrated the highest diagnostic efficacy for trastuzumab\u2010induced cardiotoxicity.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>This study confirms that GLS, GRS, and GCS can predict early cardiac dysfunction in a mouse model using 2D\u2010STE. Additionally, histological analysis further validated the overall nature of cardiac damage.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jum.16755","type":"journal-article","created":{"date-parts":[[2025,6,28]],"date-time":"2025-06-28T04:03:58Z","timestamp":1751083438000},"page":"2039-2054","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Application of 2\u2010Dimensional Speckle Tracking Technology for Trastuzumab\u2010Induced Cardiotoxicity in a New Mouse Model"],"prefix":"10.1002","volume":"44","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-5544-098X","authenticated-orcid":false,"given":"Yingxuan","family":"Tu","sequence":"first","affiliation":[{"name":"Department of Ultrasound The Fourth Hospital of Hebei Medical University  Shijiazhuang China"},{"name":"Department of Ultrasound Baoding No. 1 Central Hospital  Baoding China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huaxing","family":"Zhang","sequence":"additional","affiliation":[{"name":"Core Facilities and Centers Hebei Medical University  Shijiazhuang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4332-9821","authenticated-orcid":false,"given":"Ruoling","family":"Han","sequence":"additional","affiliation":[{"name":"Department of Ultrasound The Fourth Hospital of Hebei Medical University  Shijiazhuang China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,6,28]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21834"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.1000563"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10549-014-2836-7"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM200103153441101"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa052122"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1177\/17588359211066677"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1080\/14740338.2017.1351541"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10549-019-05453-z"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41416-020-0786-x"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2005.02.4091"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10549-019-05303-y"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10549-021-06280-x"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.echo.2014.07.012"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcmg.2012.11.017"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jacc.2020.11.020"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcvm.2022.1071249"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.3390\/medicina57121372"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ultrasmedbio.2021.07.001"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1186\/s43044-021-00214-0"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1093\/gerona\/gly139"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10554-020-01777-w"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1093\/eurheartj\/ehae574"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.110.239574"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11596-015-1423-4"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2023.06.019"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2017.06.033"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.echo.2014.01.014"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2023.115662"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ncrna.2024.02.004"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2022.115388"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2022.121207"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10557-023-07522-4"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0079543"},{"key":"e_1_2_9_35_1","first-page":"536","article-title":"Administration of trastuzumab with heart irradiation induced acute cardiotoxicity in mice","volume":"10","author":"Yi P","year":"2020","journal-title":"Am J Cancer Res"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2018.09.007"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.6004\/jnccn.2022.0030"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.21037\/tbcr-24-31"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1093\/cvr\/cvaa110"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41418-019-0372-z"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1093\/eurheartj\/ehac244"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1001\/jamacardio.2019.2952"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.echo.2011.01.015"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jacc.2014.02.536"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCIMAGING.113.000989"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1093\/ehjci\/jew005"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.3760\/cma.j.issn.1004-4477.2013.04.002"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcmg.2010.05.011"}],"container-title":["Journal of Ultrasound in Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jum.16755","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jum.16755","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jum.16755","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T11:27:51Z","timestamp":1772969271000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jum.16755"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,6,28]]},"references-count":47,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2025,11]]}},"alternative-id":["10.1002\/jum.16755"],"URL":"https:\/\/doi.org\/10.1002\/jum.16755","archive":["Portico"],"relation":{},"ISSN":["0278-4297","1550-9613"],"issn-type":[{"value":"0278-4297","type":"print"},{"value":"1550-9613","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,6,28]]},"assertion":[{"value":"2024-11-06","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-06-14","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-06-28","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}