{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T23:49:21Z","timestamp":1792108161056,"version":"4.4.0"},"reference-count":38,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2025,11,24]],"date-time":"2025-11-24T00:00:00Z","timestamp":1763942400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,11,24]],"date-time":"2025-11-24T00:00:00Z","timestamp":1763942400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100012708","name":"Foundation for the Advancement of Theoretical Physics and Mathematics","doi-asserted-by":"publisher","award":["20\u20102\u201010\u201010\u20101"],"award-info":[{"award-number":["20\u20102\u201010\u201010\u20101"]}],"id":[{"id":"10.13039\/501100012708","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Ultrasound Medicine"],"published-print":{"date-parts":[[2026,5]]},"abstract":"<jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>Colorectal cancer (CRC) is the third most common cancer globally and is frequently diagnosed at metastatic stages. As an alternative to the current standard of care, a novel high\u2010intensity\u2010focused ultrasound (HIFU)\u2010based boiling histotripsy (BH) method has the potential to provide an ultrasound (US)\u2010guided approach for non\u2010invasive mechanical fractionation of CRC metastases and enhancement of anti\u2010tumor immune response for inoperable primary CRC.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Young's modulus of the autopsy human sigmoid colon cancer was first measured using shear wave elastography. Volumetric BH lesion was then produced in the cancer sample\n                      <jats:italic>ex vivo<\/jats:italic>\n                      using a 2\u2010MHz 9\u2010element annular array (67.5\u00b0 focusing angle) targeting a 3D\u2010grid (5\u2009\u00d7\u20095 foci across 2 transverse layers with 1\u2010mm spacing) under B\u2010mode US guidance. Each focus received 150 pulses of 1\u2010ms duration (peak positive\/negative pressures and shock amplitude\n                      <jats:italic>P<\/jats:italic>\n                      +\/\n                      <jats:italic>P\/A<\/jats:italic>\n                      <jats:sub>s<\/jats:sub>\n                      \u2009=\u200991\/\u221213\/78\u2009MPa, acoustic power and initial intensity within the pulse\n                      <jats:italic>W<\/jats:italic>\n                      <jats:sub>0<\/jats:sub>\n                      \u2009=\u2009125.5\u2009W and\n                      <jats:italic>I<\/jats:italic>\n                      <jats:sub>0<\/jats:sub>\n                      \u2009=\u20092.7\u2009W\/cm\n                      <jats:sup>2<\/jats:sup>\n                      ) delivered at 0.1\u2010s intervals (1% duty cycle). The obtained lesion was analyzed\n                      <jats:italic>via<\/jats:italic>\n                      B\u2010mode imaging and histologically with hematoxylin\u2013eosin and Masson's trichrome.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Young's modulus of the autopsy CRC sample was 38\u2009\u00b1\u200910\u2009kPa and aligned with clinical values for rectal adenocarcinomas. BH treatment was successfully guided in real\u2010time using B\u2010mode US: the treated area was hyperechoic during BH and hypoechoic post\u2010treatment. Histology confirmed the presence of intact mucinous colon carcinoma outside the BH lesion, and fractionated cells and damaged stroma with disrupted collagen fibers within the lesion.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      This\n                      <jats:italic>ex vivo<\/jats:italic>\n                      pilot study demonstrates the potential of BH for non\u2010invasive non\u2010thermal US\u2010guided treatment for CRC and metastases.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jum.70137","type":"journal-article","created":{"date-parts":[[2025,11,24]],"date-time":"2025-11-24T10:45:21Z","timestamp":1763981121000},"page":"991-999","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Boiling Histotripsy as a Non\u2010Invasive Non\u2010Thermal Approach for Treatment of Human Colon Cancer Tumors"],"prefix":"10.1002","volume":"45","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6486-2895","authenticated-orcid":false,"given":"Ekaterina","family":"Ponomarchuk","sequence":"first","affiliation":[{"name":"Physics Faculty Lomonosov Moscow State University  Moscow Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sergey","family":"Tsysar","sequence":"additional","affiliation":[{"name":"Physics Faculty Lomonosov Moscow State University  Moscow Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexey","family":"Kadrev","sequence":"additional","affiliation":[{"name":"Lomonosov Moscow State University Clinic Medical Scientific and Educational Institute  Moscow Russia"},{"name":"Diagnostic Ultrasound Division Russian Medical Academy of Continuous Professional Education  Moscow Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daria","family":"Chupova","sequence":"additional","affiliation":[{"name":"Physics Faculty Lomonosov Moscow State University  Moscow Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Polina","family":"Pestova","sequence":"additional","affiliation":[{"name":"Physics Faculty Lomonosov Moscow State University  Moscow Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Karzova","sequence":"additional","affiliation":[{"name":"Physics Faculty Lomonosov Moscow State University  Moscow Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liliya","family":"Papikyan","sequence":"additional","affiliation":[{"name":"Physics Faculty Lomonosov Moscow State University  Moscow Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anastasia","family":"Kvashennikova","sequence":"additional","affiliation":[{"name":"Physics Faculty Lomonosov Moscow State University  Moscow Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natalia","family":"Danilova","sequence":"additional","affiliation":[{"name":"Lomonosov Moscow State University Clinic Medical Scientific and Educational Institute  Moscow Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pavel","family":"Malkov","sequence":"additional","affiliation":[{"name":"Lomonosov Moscow State University Clinic Medical Scientific and Educational Institute  Moscow Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrey","family":"Chernyaev","sequence":"additional","affiliation":[{"name":"Department of Fundamental Pulmonology Pulmonology Scientific Research Institute  Moscow Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sergey","family":"Buravkov","sequence":"additional","affiliation":[{"name":"Faculty of Fundamental Medicine Lomonosov Moscow State University  Moscow Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vera","family":"Khokhlova","sequence":"additional","affiliation":[{"name":"Physics Faculty Lomonosov Moscow State University  Moscow Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,11,24]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21660"},{"key":"e_1_2_8_3_1","unstructured":"World Health Organization.Colorectal cancer (2023 July 11). Accessed December 2 2024.https:\/\/www.who.int\/news-room\/fact-sheets\/detail\/colorectal-cancer."},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v30.i7.644"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-30106-4"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2016.12.033"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ultrasmedbio.2017.06.003"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1308\/147870811X592458"},{"key":"e_1_2_8_9_1","unstructured":"Online database of clinical research studies. Accessed December 2 2024.https:\/\/clinicaltrials.gov."},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijrobp.2016.03.019"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1080\/02656736.2021.1905189"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1080\/02656736.2023.2233720"},{"key":"e_1_2_8_13_1","volume-title":"Nonlinear Acoustics","author":"Hamilton M","year":"1998"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1121\/1.4788649"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ultrasmedbio.2009.09.010"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00270-022-03309-6"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1109\/TUFFC.2025.3596780"},{"key":"e_1_2_8_18_1","unstructured":"KhokhlovaVA RosnitskiyPB YuldashevPV et al.Design of a transrectal probe for boiling histotripsy ablation of prostate [abstract].18th International Symposium on Therapeutic Ultrasound. 2018;67\u201369."},{"key":"e_1_2_8_19_1","unstructured":"SchadeGR KhokhlovaTD HunterC et al.A preclinical transrectal boiling histotripsy system for prostate ablation [abstract].34rd Annual Meeting of Engineering and Urology Society (EUS). 2019;46."},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1088\/1361-6560\/aaef16"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1109\/TUFFC.2023.3261420"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1080\/02656736.2018.1438672"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0173867"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ultrasmedbio.2022.07.014"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ultras.2023.107029"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.3390\/gidisord6020026"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2021.681629"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ultrasmedbio.2024.05.002"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10517-024-06144-6"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ultrasmedbio.2024.10.006"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1134\/S1063771022010043"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1134\/S1063771023600560"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1109\/TUFFC.2021.3074611"},{"key":"e_1_2_8_34_1","unstructured":"HasgallPA Di GennaroF BaumgartnerC et al.IT'IS database for thermal and electromagnetic parameters of biological tissues. Version 4.1.2022. Accessed December 2 2024.https:\/\/itis.swiss\/virtual-population\/tissue-properties\/database."},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ultrasmedbio.2019.03.006"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.7150\/jca.12094"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers14071612"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-45542-z"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12885-022-09994-3"}],"container-title":["Journal of Ultrasound in Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jum.70137","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jum.70137","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jum.70137","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,7]],"date-time":"2026-04-07T04:03:31Z","timestamp":1775534611000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jum.70137"}},"subtitle":["An\n                    <i>Ex Vivo<\/i>\n                    Proof\u2010of\u2010Concept"],"short-title":[],"issued":{"date-parts":[[2025,11,24]]},"references-count":38,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2026,5]]}},"alternative-id":["10.1002\/jum.70137"],"URL":"https:\/\/doi.org\/10.1002\/jum.70137","archive":["Portico"],"relation":{},"ISSN":["0278-4297","1550-9613"],"issn-type":[{"value":"0278-4297","type":"print"},{"value":"1550-9613","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,11,24]]},"assertion":[{"value":"2025-06-03","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-11-03","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-11-24","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}