{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,27]],"date-time":"2026-07-27T14:55:32Z","timestamp":1785164132745,"version":"3.55.0"},"reference-count":30,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2025,12,4]],"date-time":"2025-12-04T00:00:00Z","timestamp":1764806400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2025,12,4]],"date-time":"2025-12-04T00:00:00Z","timestamp":1764806400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Ultrasound Medicine"],"published-print":{"date-parts":[[2026,5]]},"abstract":"<jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>\n                      <jats:italic>O<\/jats:italic>\n                      \u2010GlcNAcylation plays a key regulatory role in hepatic physiology, and its disruption leads to fibrosis in liver\u2010specific OGT knockout mice (OGT\n                      <jats:sup>LKO<\/jats:sup>\n                      ), making this model valuable for studying advanced metabolic dysfunction\u2010associated steatohepatitis (MASH). Our objective is to demonstrate that shear wave elastography (SWE) is a suitable non\u2010invasive tool to characterize and follow this mouse model of liver injury.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      We weekly monitored non\u2010invasively liver fibrosis progression in OGT\n                      <jats:sup>LKO<\/jats:sup>\n                      mice from 5 to 8\u2009weeks of age, using SWE on both a high\u2010frequency preclinical ultrasound system (VevoF2), and a clinical reference system (Aixplorer) and assuming the liver as homogeneous, isotropic and purely elastic. Stiffness measurements were then confronted with ex vivo liver histological scoring and major gene transcripts associated with the development of the pathology.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Weekly liver stiffness measurements demonstrated a progressive increase, correlating strongly with histological fibrosis scores (\n                      <jats:italic>R<\/jats:italic>\n                      <jats:sup>2<\/jats:sup>\n                      \u2009&gt;\u20090.78) and fibrosis\u2010related gene expression (Col3a1, Col6a1). The high\u2010resolution imaging capability of the VevoF2 allowed precise anatomical exploration while delivering SWE measurements consistent with the clinical device (Pearson\n                      <jats:italic>r<\/jats:italic>\n                      \u2009=\u20090.92).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Our study confirms that following liver stiffness fibrosis by SWE in a mouse model of liver injury is a valuable tool that correlates with ex vivo findings. This approach facilitates a better understanding of disease progression and therapeutic evaluation in preclinical models of MASH.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jum.70146","type":"journal-article","created":{"date-parts":[[2025,12,4]],"date-time":"2025-12-04T13:40:28Z","timestamp":1764855628000},"page":"1069-1082","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Liver Stiffness Characterization of\n                    <scp>\n                      OGT\n                      <sup>LKO<\/sup>\n                    <\/scp>\n                    Mouse Model of Progressive Liver Fibrosis"],"prefix":"10.1002","volume":"45","author":[{"given":"Gilles","family":"Renault","sequence":"first","affiliation":[{"name":"Universit\u00e9 Paris Cit\u00e9 CNRS, Inserm, Institut Cochin  Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lenin","family":"Chinchilla","sequence":"additional","affiliation":[{"name":"Laboratoire d'Imagerie Biom\u00e9dicale Multimodales \u00e0 Paris\u2010Saclay, BioMaps Universit\u00e9 Paris\u2010Saclay  Orsay France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lourdes","family":"Massengo","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Paris Cit\u00e9 CNRS, Inserm, Institut Cochin  Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pierre","family":"Monbernard","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Paris Cit\u00e9 CNRS, Inserm, Institut Cochin  Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fadila","family":"Benhamed","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Paris Cit\u00e9 CNRS, Inserm, Institut Cochin  Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5005-0597","authenticated-orcid":false,"given":"Marion","family":"Regnier","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Paris Cit\u00e9 CNRS, Inserm, Institut Cochin  Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7677-7460","authenticated-orcid":false,"given":"Raphael","family":"Denis","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Paris Cit\u00e9 CNRS, Inserm, Institut Cochin  Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catherine","family":"Postic","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Paris Cit\u00e9 CNRS, Inserm, Institut Cochin  Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anthony","family":"Novell","sequence":"additional","affiliation":[{"name":"Laboratoire d'Imagerie Biom\u00e9dicale Multimodales \u00e0 Paris\u2010Saclay, BioMaps Universit\u00e9 Paris\u2010Saclay  Orsay France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean\u2010Luc","family":"Gennisson","sequence":"additional","affiliation":[{"name":"Laboratoire d'Imagerie Biom\u00e9dicale Multimodales \u00e0 Paris\u2010Saclay, BioMaps Universit\u00e9 Paris\u2010Saclay  Orsay France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,12,4]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.eprac.2022.03.010"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1136\/gutjnl-2020-322786"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.metabol.2024.155914"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.AW119.003226"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhepr.2023.100878"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmri.28765"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ultrasmedbio.2011.05.016"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2014.09.020"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ultrasmedbio.2008.08.018"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.58688"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.7150\/thno.34066"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep4.1912"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/liv.16141"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1136\/gutjnl-2014-308969"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1109\/TUFFC.2004.1295425"},{"key":"e_1_2_8_17_1","volume-title":"Elastic Waves in Solids: I. 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