{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,14]],"date-time":"2026-07-14T13:35:48Z","timestamp":1784036148480,"version":"3.55.0"},"reference-count":39,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2019,10,22]],"date-time":"2019-10-22T00:00:00Z","timestamp":1571702400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Kaohsiung J of Med Scie"],"published-print":{"date-parts":[[2020,1]]},"abstract":"<jats:p>Accumulating evidences have proved the protective role of traditional Chinese medicine in improving neurological damage induced by cerebral hypoxia\u2010ischemia. Herein, we hypothesized that <jats:italic>Dendrobium officinale<\/jats:italic> aqueous extract exerted neuroprotection against brain damage. Initially, a model of hypoxic\u2010ischemic brain damage (HIBD) was induced in neonatal rats, which were subsequently intragastrically administered with different doses of <jats:italic>Dendrobium officinale<\/jats:italic> aqueous extract. Next, the antioxidant capacity was examined by enzyme\u2010linked immunosorbent assay. 2,3,5\u2010Triphenyltetrazolium chloride and terminal deoxynucleotidyl transferase\u2010mediated dUTP nick end\u2010labeling staining assays were adopted to determine neuronal apoptosis in brain tissues. Furthermore, neurotrophic factors and hypoxia\u2010inducible factor\u20101\u03b1 (HIF\u20101\u03b1) expression was identified by Western blot analysis. The neonatal rat models of HIBD presented impaired neurobehaviors and antioxidant capacity, increased neuronal apoptosis and expression of HIF\u20101\u03b1 and histone deacetylase 1 (HDAC1), as well as diminished expression of neurotrophic factors and K<jats:sup>+<\/jats:sup>\u2010Cl<jats:sup>\u2212<\/jats:sup>\u2010cotransporter 2 (KCC2). Notably, in response to different doses of <jats:italic>Dendrobium officinale<\/jats:italic> aqueous extract, the impairment on neurobehaviors and antioxidant capacity was alleviated, accompanied by reduced levels of nitric oxide synthase, nitric oxide, and malondialdehyde, and increased superoxide dismutase activity. Besides, the neuronal apoptosis was inhibited as reflected by down\u2010regulated cleaved caspase\u20103 and Bax and up\u2010regulated Bcl\u20102. Moreover, we also found accelerated expression of neurotrophic factors and KCC2 and diminished expression of HIF\u20101\u03b1 and HDAC1. Altogether, this present study highlights that the aqueous extract of <jats:italic>Dendrobium officinale<\/jats:italic> can suppress the neuronal apoptosis and enhance the expression of neurotrophic factors to protect neonatal rats against HIBD.<\/jats:p>","DOI":"10.1002\/kjm2.12139","type":"journal-article","created":{"date-parts":[[2019,10,23]],"date-time":"2019-10-23T06:56:18Z","timestamp":1571813778000},"page":"43-53","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Aqueous extract of <i>Dendrobium officinale<\/i> confers neuroprotection against hypoxic\u2010ischemic brain damage in neonatal rats"],"prefix":"10.1002","volume":"36","author":[{"given":"Xiao\u2010Li","family":"Li","sequence":"first","affiliation":[{"name":"Department of Paediatrics Affiliated Hangzhou First People's Hospital, Zhejiang University School of Medicine  Hangzhou Zhejiang PR 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