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We analyzed the target genes of the differentially expressed (DE) miRNAs to analyze the function and pathway enrichment using Gene Ontology and Kyoto Encyclopedia of Genes and Genomes, respectively. We validated five candidate miRNAs by real time\u2010qPCR. Integrated miRNA\u2010mRNA\u2010pathway network analysis was performed to show the interaction network of the candidate miRNAs and their target genes of interest with the involved pathways. It was observed that 227 miRNAs were differently expressed between the MA group and the early pregnancy control group, with 58 miRNAs downregulated and 169 miRNAs upregulated in the MA group. Real\u2010time qPCR results revealed that expression of the five candidate miRNAs, namely hsa\u2010miR\u201022\u20103p, hsa\u2010miR\u2010145\u20103p, hsa\u2010miR\u2010107, hsa\u2010miR\u2010361\u20103p, and hsa\u2010miR\u2010378c, was consistent with the miRNA data obtained by sequencing. Integrated miRNA\u2010mRNA\u2010pathway network analysis illustrated that target genes of the candidate miRNAs were mainly involved in the PI3K\u2010Akt signaling pathway, HIF\u20101 signaling pathway, and VEGF signaling pathway, which would have potential significance in pregnancy and MA. We are the first to reveal the DE miRNAs involved in MA and illustrate their functional interaction network. These results might provide potential circulating biomarkers and new therapeutic targets for MA.<\/jats:p>","DOI":"10.1002\/kjm2.12144","type":"journal-article","created":{"date-parts":[[2019,11,5]],"date-time":"2019-11-05T16:33:30Z","timestamp":1572971610000},"page":"114-121","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Comprehensive analysis of the differential expression profile of microRNAs in missed abortion"],"prefix":"10.1002","volume":"36","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0717-0929","authenticated-orcid":false,"given":"Qiao\u2010Xian","family":"Tian","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynecology The Second Affiliated Hospital of Soochow University  Suzhou Jiangsu P.R. China"},{"name":"Department of Obstetrics and Gynecology Fengcheng Hospital  Fengxian District Shanghai P.R. 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