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In the study, we aimed to investigate the effects and mechanisms of miR\u2010326 on airway remodeling in airway smooth muscle cells (ASMCs). The results showed that transforming growth factor\u2010\u03b21 (TGF\u2010\u03b21) accelerated matrix protein deposition by increasing the expression levels of collagen I and fibronectin, and promoted proliferative ability of ASMCs. However, miR\u2010326 was significantly downregulated in TGF\u2010\u03b21\u2010treated ASMCs. MiR\u2010326 mimics robustly decreased the collagen I and fibronectin levels and inhibited cell proliferation of TGF\u2010\u03b21\u2010treated ASMCs. Luciferase assay investigated that tumor necrosis factor superfamily member 14 (TNFSF14) was a direct target of miR\u2010326. The expression of TNFSF14 was negatively regulated by miR\u2010326. Moreover, exogenous TNFSF14 effectively reversed the inhibitory effects of miR\u2010326 overexpression on the expression levels of collagen I and fibronectin, and promoted cell proliferation of TGF\u2010\u03b21\u2010treated ASMCs. In conclusion, miR\u2010326 suppressed matrix protein deposition and cell proliferation of TGF\u2010\u03b21\u2010treated ASMCs via inhibiting TNFSF14. MiR\u2010326 might be a promising novel therapeutic target for asthma.<\/jats:p>","DOI":"10.1002\/kjm2.12197","type":"journal-article","created":{"date-parts":[[2020,3,2]],"date-time":"2020-03-02T14:12:30Z","timestamp":1583158350000},"page":"508-514","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["TNFSF14, a novel target of miR\u2010326, facilitates airway remodeling in airway smooth muscle cells via inducing extracellular matrix protein deposition and proliferation"],"prefix":"10.1002","volume":"36","author":[{"given":"Hui","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Pediatric Affiliated Hospital of Changchun University of Chinese Medicine  Changchun Jilin Province China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huan\u2010Li","family":"Yan","sequence":"additional","affiliation":[{"name":"Department of Neonatology The Second People's Hospital of Liaocheng  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