{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T23:09:15Z","timestamp":1790723355767,"version":"4.1.0"},"reference-count":39,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2020,5,12]],"date-time":"2020-05-12T00:00:00Z","timestamp":1589241600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Kaohsiung J of Med Scie"],"published-print":{"date-parts":[[2020,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Inflammatory bowel disease (IBD) is characterized by recurring inflammatory disorders in digestive system, and devoid of effective treatment. Proprotein convertase subtilisin\/kexin type 9 (PCSK9), stimulated via inflammation whose inhibition could decrease secretion of inflammatory factors. We then determined whether inhibition of PCSK9 could improve the inflammation. First, rats model of colitis was first established via administration of 2,4,6\u2010trinitrobenzenesulfonic acid (TNBS), and then verified via determination of body weight loss, myeloperoxidase (MPO) activity, and histopathological analysis of colonic damage. Results showed that treatment with TNBS induced a great body weight loss, MPO activity increase, and serious colonic damage, showing an obviously character of IBD. PCSK9 was elevated in TNBS\u2010induced rats, and PCSK9 inhibition delivered by adenovirus vector increased the body weight, decreased MPO activity, and ameliorated histological change of colon. Second, the protective effect of PCSK9 inhibition against TNBS\u2010induced colitis was accompanied by decrease of proinflammatory factors secretion, including tumor necrosis factor\u2010\u03b1, interleukin\u20101\u03b2, interleukin\u20106, intercellular adhesion molecule 1, and monocyte chemoattractant protein\u20101. TNBS could activate toll\u2010like receptor 4 (TLR4)\/nuclear factor\u2010kappa B (NF\u2010\u03baB) signaling pathway, while PCSK9 inhibition suppressed activation of TLR4\/NF\u2010\u03baB in TNBS\u2010induced rats. In conclusion, PCSK9 inhibition attenuated TNBS\u2010induced rat colitis through anti\u2010inflammatory effect under inactivation of TLR4\/NF\u2010\u03baB, suggesting potential therapeutic strategy in IBD.<\/jats:p>","DOI":"10.1002\/kjm2.12225","type":"journal-article","created":{"date-parts":[[2020,5,12]],"date-time":"2020-05-12T18:39:51Z","timestamp":1589308791000},"page":"705-711","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["Inhibition of proprotein convertase subtilisin\/kexin type 9 attenuates 2,4,6\u2010trinitrobenzenesulfonic acid\u2010induced colitis via repressing toll\u2010like receptor 4\/nuclear factor\u2010kappa B"],"prefix":"10.1002","volume":"36","author":[{"given":"Lei","family":"Lei","sequence":"first","affiliation":[{"name":"GI Medicine The Central Hospital of Enshi Autonomous Prefecture  Enshi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xu","family":"Li","sequence":"additional","affiliation":[{"name":"Cardiothoracic Surgery The Central Hospital of Enshi Autonomous Prefecture  Enshi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"You\u2010Jun","family":"Yuan","sequence":"additional","affiliation":[{"name":"Department of Emergency WenZhou Central Hospital  Wenzhou City China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhi\u2010Li","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Emergency WenZhou Central Hospital  Wenzhou City China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian\u2010Hua","family":"He","sequence":"additional","affiliation":[{"name":"GI Medicine The Central Hospital of Enshi Autonomous Prefecture  Enshi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian\u2010Hua","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Emergency WenZhou Central Hospital  Wenzhou City China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8542-4253","authenticated-orcid":false,"given":"Xiao\u2010Sheng","family":"Cai","sequence":"additional","affiliation":[{"name":"Department of Emergency WenZhou Central Hospital  Wenzhou City China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,5,12]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1097\/MIB.0000000000000726"},{"issue":"02","key":"e_1_2_7_3_1","first-page":"102","article-title":"Curcumin improves TNBS\u2010induced colitis in rats by inhibiting IL\u201027 expression via the TLR4\/NF\u2010kappa B signaling pathway","volume":"29","author":"Zeng Z","year":"2013","journal-title":"Planta Med"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/mnfr.201300771"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1097\/MOL.0b013e32834622b5"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrendo.2012.254"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.5114\/aoms.2019.84734"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2008.07.023"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1097\/SHK.0000000000000682"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jix574"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0123685"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.ATV.0000160340.72641.87"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2014.08.024"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.atherosclerosis.2017.04.023"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/ibd.21282"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2567.2004.01976.x"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jff.2018.07.005"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2011.10.001"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms19103057"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.autrev.2017.02.013"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1124\/jpet.112.201863"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1124\/jpet.114.218750"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1101736"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0813036106"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/nsmb1235"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-20425-x"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1093\/eurheartj\/ehx260"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00133.2015"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.7150\/thno.20359"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1616447113"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2017.11.101"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.3390\/vaccines4010005"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.diff.2019.01.002"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.3892\/ijmm.2013.1458"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2249.2007.03531.x"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00418-005-0092-y"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10787-016-0263-6"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.90496.2008"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2796.2008.01953.x"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10787-018-0523-8"}],"container-title":["The Kaohsiung Journal of Medical Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fkjm2.12225","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/kjm2.12225","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/kjm2.12225","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/kjm2.12225","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,5]],"date-time":"2023-09-05T22:13:48Z","timestamp":1693952028000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/kjm2.12225"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,5,12]]},"references-count":39,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2020,9]]}},"alternative-id":["10.1002\/kjm2.12225"],"URL":"https:\/\/doi.org\/10.1002\/kjm2.12225","archive":["Portico"],"relation":{},"ISSN":["1607-551X","2410-8650"],"issn-type":[{"value":"1607-551X","type":"print"},{"value":"2410-8650","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,5,12]]},"assertion":[{"value":"2020-01-02","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-04-16","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-05-12","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}