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After wild\u2010type (WT) and miR\u2010155<jats:sup>\u2212\/\u2212<\/jats:sup> mice were injected with complete Freund's adjuvant and prostate antigen to establish EAP model, half were randomly selected for injection with lipopolysaccharide (LPS, a TLR4 ligand). The following experiments were then performed: von Frey filaments, hematoxylin\u2010eosin (HE) staining, real time quantitative polymerase chain reaction (qRT\u2010PCR), Western blotting, and enzyme\u2010linked immunosorbent assay (ELISA). And the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH\u2010Px) and the level of Malondialdehyde (MDA) were detected by corresponding kits.miR\u2010155<jats:sup>\u2212\/\u2212<\/jats:sup> mice with prostatitis exhibited the attenuated pelvic tactile allodynia\/hyperalgesia and the suppressed TLR4\/nuclear factor\u2010kappa B (NF\u2010\u03baB) pathway as compared with the WT mice with prostatitis. In addition, LPS enhanced the upregulation of miR\u2010155 and the activation of the TLR4\/NF\u2010\u03baB pathway in the prostatic tissues of WT mice with EAP. Furthermore, prostatitis mice had aggravated inflammation scores accompanying the increased interleukin (IL)\u20101\u03b2, tumor necrosis factor\u2010\u03b1, IL\u20106, interferon\u2010\u03b3, IL\u201012, and MDA in prostatic tissues with the decreased IL\u201010, SOD and GSH\u2010Px, and the unaltered IL\u20104. Compared with the mice from the WT\u2009+\u2009EAP group and the miR\u2010155<jats:sup>\u2212\/\u2212<\/jats:sup> +\u2009EAP\u2009+\u2009LPS group, mice from the miR\u2010155<jats:sup>\u2212\/\u2212<\/jats:sup> +\u2009EAP group had decreased inflammation and oxidative stress. miR\u2010155 deficiency ameliorated pelvic tactile allodynia\/hyperalgesia in EAP mice and improved inflammation and oxidative stress in prostatic tissues in a TLR4\u2010dependent manner involving NF\u2010\u03baB activation, thereby exerting a therapeutic effect in chronic prostatitis treatment.<\/jats:p>","DOI":"10.1002\/kjm2.12229","type":"journal-article","created":{"date-parts":[[2020,5,21]],"date-time":"2020-05-21T08:14:01Z","timestamp":1590048841000},"page":"712-720","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["<scp>MicroRNA<\/scp>\u2010155 deficiency attenuates inflammation and oxidative stress in experimental autoimmune prostatitis in a <scp>TLR4<\/scp>\u2010dependent manner"],"prefix":"10.1002","volume":"36","author":[{"given":"Xian","family":"Fu","sequence":"first","affiliation":[{"name":"Department of Urology, Affiliated Hangzhou First People's Hospital, School of Medicine Zhejiang University  Zhejiang Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hua\u2010Dong","family":"He","sequence":"additional","affiliation":[{"name":"Department of Urology, Affiliated Hangzhou First People's Hospital, School of Medicine Zhejiang University  Zhejiang Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chang\u2010Jiu","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Urology Nanjing Medical University  Nanjing Jiangsu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ning","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Urology, Affiliated Hangzhou First People's Hospital, School of 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