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The hypertrophy and hyperplasia of airway smooth muscle cells (ASMCs) are hallmarks of bronchial remodeling in asthma. In this study, the regulatory effects of microRNA\u2010620 (miR\u2010620) on ASMC proliferation and apoptosis in response to transforming growth factor \u03b21 (TGF\u2010\u03b21) stimulation was investigated. The expression of miR\u2010620 was significantly upregulated in TGF\u2010\u03b21\u2010treated ASMCs compared with vehicle\u2010treated cells. Downregulation of miR\u2010620 suppressed the proliferation and increased apoptosis in TGF\u2010\u03b21\u2010stimulated ASMCs. Phosphatase and tensin homolog (PTEN) was predicted and confirmed as a downstream target of miR\u2010620. PTEN was upregulated in miR\u2010620\u2010inhibitor transfected ASMCs, but decreased in cells delivered with miR\u2010620 mimics. Moreover, knocking down miR\u2010620 alone efficiently reduced the phosphorylation of protein kinase B (AKT), decreased TGF\u2010\u03b21\u2010induced proliferation and promoted apoptosis in ASMCs, whereas downregulation of PTEN in miR\u2010620 inhibitor\u2010transfected cells restored the activation of AKT, increased TGF\u2010\u03b21\u2010triggered proliferation, and partially inhibited ASMC apoptosis. Taken together, the present study provided evidence that miR\u2010620 increased TGF\u2010\u03b21\u2010mediated proliferation and suppressed apoptosis in ASMCs via the regulation of PTEN and AKT expression. These findings suggest that miR\u2010620\/PTEN\/AKT axis may be considered as a therapeutic target for asthma treatment.<\/jats:p>","DOI":"10.1002\/kjm2.12260","type":"journal-article","created":{"date-parts":[[2020,6,25]],"date-time":"2020-06-25T06:28:40Z","timestamp":1593066520000},"page":"869-877","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["<scp>MiRNA<\/scp>\u2010620 promotes <scp>TGF<\/scp>\u2010\u03b21\u2010induced proliferation of airway smooth muscle cell through controlling <scp>PTEN<\/scp>\/<scp>AKT<\/scp> signaling pathway"],"prefix":"10.1002","volume":"36","author":[{"given":"Hui","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Pediatrics Second Affiliated Hospital of Tianjin University of Traditional Chinese Medicine  Tianjin City 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