{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T19:42:57Z","timestamp":1790797377571,"version":"4.1.0"},"reference-count":38,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2021,2,21]],"date-time":"2021-02-21T00:00:00Z","timestamp":1613865600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Kaohsiung J of Med Scie"],"published-print":{"date-parts":[[2021,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A range of circular (Circ) RNAs have been demonstrated to be of therapeutic significance for the treatment of acute lymphoblastic leukemia (ALL). Here, we investigated the mechanisms underlying the action of Circ\u2010PRKDC and the microRNA\u2010653\u20105p\/Reelin (miR\u2010653\u20105p\/RELN) axis in T\u2010cell ALL (T\u2010ALL).Clinical specimens were obtained from patients with T\u2010ALL (n\u00a0=\u00a039) and healthy controls (n\u00a0=\u00a030). In each specimen, we determined the expression levels of Circ\u2010PRKDC, miR\u2010653\u20105p, and RELN. Human T\u2010ALL cells (Jurkat) were transfected with Circ\u2010PRKDC\u2010 or miR\u2010653\u20105p\u2010related sequences to investigate cell proliferation, apoptosis, and autophagy. We also determined the levels of Circ\u2010PRKDC, miR\u2010653\u20105p, RELN, and signaling proteins related to phosphoinositide 3\u2010kinase (PI3K), AKT, and mammalian target of rapamycin (mTOR). Finally, we decoded the interactions between Circ\u2010PRKDC, miR\u2010653\u20105p, and RELN. The expression levels of Circ\u2010PRKDC and RELN were upregulated in T\u2010ALL tissues and cells while the levels of miR\u2010653\u20105p were downregulated. Thereafter, then silencing of Circ\u2010PRKDC, or the enforced expression of miR\u2010653\u20105p, repressed the expression of RELN and the activation of the PI3K\/AKT\/mTOR signaling pathway, thus enhancing cell autophagy and apoptosis, and disrupting cell proliferation. Circ\u2010PRKDC acted a sponge for miR\u2010653\u20105p while miR\u2010653\u20105p targeted RELN. The knockdown of miR\u2010653\u20105p abrogated the silencing of Circ\u2010PRKDC\u2010induced effects in T\u2010ALL cells. The depletion of Circ\u2010PRKDC elevated miR\u2010653\u20105p to silence RELN\u2010mediated PI3K\/AKT\/mTOR signaling activation, thereby enhancing autophagy and apoptosis in T\u2010ALL cells.<\/jats:p>","DOI":"10.1002\/kjm2.12352","type":"journal-article","created":{"date-parts":[[2021,2,22]],"date-time":"2021-02-22T06:36:24Z","timestamp":1613975784000},"page":"392-401","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["The depletion of <scp>Circ\u2010PRKDC<\/scp> enhances autophagy and apoptosis in T\u2010cell acute lymphoblastic leukemia via <scp>microRNA<\/scp>\u2010653\u20105p\/Reelin mediation of the <scp>PI3K<\/scp>\/<scp>AKT<\/scp>\/<scp>mTOR<\/scp> signaling pathway"],"prefix":"10.1002","volume":"37","author":[{"given":"Zhang","family":"Ling","sequence":"first","affiliation":[{"name":"Department of Hematology The Third Affiliated Hospital of Sun Yat\u2010sen University  Guangzhou Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhi\u2010Gang","family":"Fang","sequence":"additional","affiliation":[{"name":"Department of Hematology The Third Affiliated Hospital of Sun Yat\u2010sen University  Guangzhou Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jie\u2010Yong","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Hematology The Third Affiliated Hospital of Sun Yat\u2010sen University  Guangzhou Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7878-3680","authenticated-orcid":false,"given":"Jia\u2010Jun","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Hematology The Third Affiliated Hospital of Sun Yat\u2010sen University  Guangzhou Guangdong 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