{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T15:48:10Z","timestamp":1777564090520,"version":"3.51.4"},"reference-count":36,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2021,5,6]],"date-time":"2021-05-06T00:00:00Z","timestamp":1620259200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Kaohsiung J of Med Scie"],"published-print":{"date-parts":[[2021,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>This study aims to investigate if the radiosensitivity of non\u2010small cell lung cancer (NSCLC) cells can be regulated by long noncoding RNA (lncRNA) colon cancer associated transcript1 (CCAT1). CCAT1 was detected by quantitative reverse transcription\u2010polymerase chain reaction (qRT\u2010PCR) in NSCLC cells (A549, H1299, SK\u2010MES1, H460, and H647) and human bronchial epithelial cells (16HBE). H460 and A549 cells were then selected for the determination of CCAT1 expression after exposure to radiation (0, 2, 4, 6\u00a0Gy) at different time points (0, 6, 12, 24\u2009h). Colony forming assay was performed to evaluate the effects of CCAT1 siRNA or pcDNA3.1\u2010CCAT1 vector on the radiosensitivity of H460 and A549 cells. Then, flow cytometry, western blotting and qRT\u2010PCR were also conducted. CCAT1 was increased in NSCLC cells when compared with 16HBE cells, which was declined in a time\u2010 and dosage\u2010dependent manner after exposure to radiation. The H460 and A549 cell colonies were decreased and the \u03b3\u2010H2AX expression was elevated with the increase of radiation dosage, which was more obvious in those transfected with CCAT1 siRNA. CCAT1 downregulation arrested NSCLC cells at G2\/M phase. Moreover, the enhanced apoptosis of radiotherapy\u2010treated NSCLC cells with reductions of p\u2010p38\/p38, p\u2010ERK\/ERK, and p\u2010JNK\/JNK was promoted by siCCAT1, but it was reversed by pcDNA3.1\u2010CCAT1 vector. Inhibiting CCAT1 regulated cell cycle, DNA damage and apoptosis of NSCLC cells, and affected MAPK pathway, eventually improving the radiosensitivity of NSCLC.<\/jats:p>","DOI":"10.1002\/kjm2.12387","type":"journal-article","created":{"date-parts":[[2021,5,6]],"date-time":"2021-05-06T21:23:17Z","timestamp":1620336197000},"page":"654-663","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["<scp>LncRNA CCAT1<\/scp> downregulation increases the radiosensitivity of non\u2010small cell lung cancer cells"],"prefix":"10.1002","volume":"37","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5122-343X","authenticated-orcid":false,"given":"Zhao","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Oncology Radiotherapy 2 Yantai Yantaishan Hospital  Yantai Shandong 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