{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T08:18:40Z","timestamp":1780474720124,"version":"3.54.1"},"reference-count":38,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2021,7,2]],"date-time":"2021-07-02T00:00:00Z","timestamp":1625184000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Kaohsiung J of Med Scie"],"published-print":{"date-parts":[[2021,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>To explore the role of suppressor of cytokine signaling\u20103 (SOCS\u20103) in mesangial proliferative glomerulonephritis (MsPGN). SOCS\u20103 expression in kidney tissues from MsPGN patients was detected using immunohistochemistry. Double immunofluorescence staining was performed to investigate the localization of SOCS\u20103 with \u03b1\u2010SMA in glomeruli. Heminephrectomized wild\u2010type (WT) and SOCS\u20103<jats:sup>\u2212\/\u2212<\/jats:sup> (KO) mice were injected with Habu\u2010snake venom (HSV) to establish MsPGN models, and renal function were compared. Simultaneously, immunofluorescence, periodic acid\u2010Schiff staining, Picrosirius red staining, as well as immunohistochemistry for PCNA, MAC\u20102 and type IV collagen in glomeruli were performed. In addition, primary mouse renal mesangial cells and SV40 MES\u201013 cells were transfected with SOCS\u20103 siRNA or SOCS\u20103 lentiviral activation particles, followed by EdU assay, flow cytometry, quantitative reverse transcription\u2010polymerase chain reaction, and Western blotting. Mesangial SOCS\u20103 expression was enhanced in glomeruli of MsPGN patients, and SOCS\u20103 was well co\u2010localized with activated \u03b1\u2010SMA. After HSV injection, WT and KO mice presented with the increases in the serum creatinine, urea nitrogen, and urinary protein, especially in KO mice. Besides, SOCS\u20103<jats:sup>\u2212\/\u2212<\/jats:sup> alleviated the hyperplasia of glomerular MCs in MsPGN mice, with the reductions in PCNA, MAC\u20102, and collagen deposition. Furthermore, SOCS\u20103 inhibition reduced the cell proportion at S phase to suppress cell proliferation, with the downregulations of Cyclin A, Cyclin D1, PCNA, and Ki\u201067. SOCS\u20103 knockout can alleviate the hyperplasia of glomerular MCs in MsPGN mice via affecting the cell cycle and proliferation of MCs, thus being a potential therapeutic target for MsPGN.<\/jats:p>","DOI":"10.1002\/kjm2.12415","type":"journal-article","created":{"date-parts":[[2021,7,2]],"date-time":"2021-07-02T10:32:14Z","timestamp":1625221934000},"page":"872-882","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Inhibition of suppressor of cytokine signaling\u20103 affects mesangial cell proliferation and cell cycle in mesangioproliferative glomerulonephritis"],"prefix":"10.1002","volume":"37","author":[{"given":"Min","family":"Liu","sequence":"first","affiliation":[{"name":"Department of Nephrology Wuhan Asia General Hospital  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ya\u2010Chao","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Nephrology Wuhan Asia General Hospital  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6024-0511","authenticated-orcid":false,"given":"Jun","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Paediatrics Xiaogan Central Hospital Affiliated with Wuhan University of Science and Technology  Xiaogan Hubei China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,7,2]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexmp.2019.104280"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2019.00978"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.5483\/BMBRep.2020.53.12.161"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2020.558341"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetimm.2011.08.002"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00467-009-1163-4"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2004050374"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1097\/MD.0000000000021372"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10067-019-04467-8"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/imm.12173"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.172.11.6969"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1159\/000447876"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1097\/00019606-200306000-00007"},{"issue":"8","key":"e_1_2_6_15_1","first-page":"1191","article-title":"Experimental mesangioproliferative glomerulonephritis in rats induced by intravenous administration of anti\u2010thymocyte serum","volume":"36","author":"Ishizaki M","year":"1986","journal-title":"Acta Pathol Jpn"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00795-011-0559-y"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0122773"},{"key":"e_1_2_6_18_1","unstructured":"In: the editor Guide for the Care and Use of Laboratory Animals. 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