{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T22:29:32Z","timestamp":1785796172984,"version":"3.56.0"},"reference-count":40,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2021,12,28]],"date-time":"2021-12-28T00:00:00Z","timestamp":1640649600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81874453"],"award-info":[{"award-number":["81874453"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004607","name":"Natural Science Foundation of Guangxi Province","doi-asserted-by":"publisher","award":["2020JJA140299"],"award-info":[{"award-number":["2020JJA140299"]}],"id":[{"id":"10.13039\/501100004607","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Kaohsiung J of Med Scie"],"published-print":{"date-parts":[[2022,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The specific mechanism of gingerol in cerebral ischemia remains unknown. A neuroprotective function for miR\u2010210 in cerebral ischemia has been identified. The brain\u2010derived neurotrophic factor (BDNF)\u2010mediated signaling pathway protects against cerebral ischemic injury. This investigation aimed to determine whether gingerol plays a neuroprotective role in cerebral ischemia via the miR\u2010210\/BDNF axis. N2a cells subjected to 10\u2009h of hypoxia and 4\u2009h of reoxygenation were treated with 5, 10, or 20\u2009\u03bcmol\/L gingerol. The levels of viability, apoptosis, and proteins in N2a cells were determined using MTT assays, flow cytometry, and western blotting, respectively. The binding relationship between BDNF and miR\u2010210 was studied using a dual luciferase reporter assay. The expression levels of miR\u2010210 and BDNF were determined using qPCR. Gingerol repressed the increase in apoptosis and decrease in viability observed in response to hypoxia\/reoxygenation. Gingerol increased Bcl\u20102, BDNF, and TrkB levels and reduced Bax and cleaved caspase 3 levels after hypoxia\/reoxygenation. Gingerol evoked decreased expression of miR\u2010210. Inhibition of miR\u2010210 resulted in increased viability and reduced apoptosis along with increased levels of Bcl\u20102, BDNF, and TrkB and reduced levels of Bax and cleaved caspase 3 after hypoxia\/reoxygenation. Additionally, the miR\u2010210 mimic reversed changes induced by gingerol. The cotransfection of the miR\u2010210 mimic and wild type BDNF led to decreased luciferase activity. BDNF was negatively regulated by miR\u2010210. BDNF siRNA reversed these changes evoked by miR\u2010210 inhibition. Gingerol ameliorated hypoxia\/reoxygenation\u2010stimulated neuronal damage by regulating the miR\u2010210\/BDNF axis, indicating that gingerol is worthy of further application in cerebral ischemia therapy.<\/jats:p>","DOI":"10.1002\/kjm2.12486","type":"journal-article","created":{"date-parts":[[2021,12,28]],"date-time":"2021-12-28T11:34:29Z","timestamp":1640691269000},"page":"367-377","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Gingerol ameliorates neuronal damage induced by <scp>hypoxia\u2010reoxygenation<\/scp> via the <scp>miR<\/scp>\u2010210\/<scp>brain\u2010derived<\/scp> neurotrophic factor axis"],"prefix":"10.1002","volume":"38","author":[{"given":"Yang","family":"Zhai","sequence":"first","affiliation":[{"name":"Graduate School Guangxi University of Chinese Medicine  Nanning China"},{"name":"Department of Neurology Guangxi Key Laboratory of Chinese Medicine Foundation Research  Nanning 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