{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,11]],"date-time":"2026-01-11T01:32:29Z","timestamp":1768095149456,"version":"3.49.0"},"reference-count":33,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2021,12,21]],"date-time":"2021-12-21T00:00:00Z","timestamp":1640044800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004737","name":"National Health Research Institutes","doi-asserted-by":"publisher","award":["NHRI\u2010110A1\u2010MRCO\u201003212101"],"award-info":[{"award-number":["NHRI\u2010110A1\u2010MRCO\u201003212101"]}],"id":[{"id":"10.13039\/501100004737","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Kaohsiung J of Med Scie"],"published-print":{"date-parts":[[2022,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Dengue virus (DENV) infection results mostly from the bites of virus\u2010carrying Aedes mosquitoes, which results in dengue fever (DF) with or without warning signs, severe dengue, or asymptomatic infections in humans. For point\u2010of care identification of DENV\u2010infected patients, a rapid diagnostic test (RDT) for DENV nonstructural protein 1 (NS1) has been developed to achieve early diagnosis and timely clinical management. We evaluated the performance of a new commercially available dengue NS1 RDT AsiaGen Dengue NS1 Antigen Rapid Diagnosis Test using real\u2010time qRT\u2010PCR as a reference method and compared the results with SD BIOLINE Dengue NS1 Ag using a single acute\u2010phase serum panel collected during the largest dengue outbreak in the history of Taiwan in 2015. The results suggested that the sensitivity and specificity of AsiaGen Dengue NS1 Antigen RDT (96.9% and 100%) were similar to those of SD BIOLINE Dengue NS1 RDT (100% and 100%) for detection in the acute phase of DENV\u20102 infection. The results suggested that the sensitivity of both RDTs was similar (95.4%\u2009~\u2009100%) for the sera collected at less than or equal to three days postsymptom onset (PSO). Our results suggested that the two DENV NS1 RDTs used in this study were promising for the timely diagnosis of DENV infection during dengue outbreaks, at least for DENV\u20102 in areas where authorized medical laboratories are not available or medical resources are limited. However, the performance of AsiaGen DENV NS1 RDTs in the detection of primary\/secondary infections and infection by serotypes of DENV other than DENV\u20102 requires further investigation.<\/jats:p>","DOI":"10.1002\/kjm2.12490","type":"journal-article","created":{"date-parts":[[2021,12,21]],"date-time":"2021-12-21T11:39:31Z","timestamp":1640086771000},"page":"385-389","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Evaluation of a new <scp>NS1<\/scp> rapid diagnostic test using a single acute\u2010phase serum panel collected during the largest dengue outbreak in Taiwan history in 2015"],"prefix":"10.1002","volume":"38","author":[{"given":"Li\u2010Teh","family":"Liu","sequence":"first","affiliation":[{"name":"Department of Medical Laboratory Science and Biotechnology College of Medical Technology, Chung\u2010Hwa University of Medical Technology  Tainan City 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