{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,13]],"date-time":"2025-05-13T15:29:19Z","timestamp":1747150159275,"version":"3.40.5"},"reference-count":38,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2022,3,24]],"date-time":"2022-03-24T00:00:00Z","timestamp":1648080000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81101058","81901759"],"award-info":[{"award-number":["81101058","81901759"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Kaohsiung J of Med Scie"],"published-print":{"date-parts":[[2022,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Treatment of myocardial infarction (MI) remains a major challenge. The chemokine family plays an important role in cardiac injury, repair, and remodeling following MI, while stromal cell\u2010derived factor\u20101 alpha (SDF\u20101\u03b1) is the most promising therapeutic target. This study aimed to increase SDF\u20101\u03b1 expression using a novel gene delivery system and further explore its effect on MI treatment. In this study, two kinds of plasmids, human SDF\u20101\u03b1 plasmid (phSDF\u20101\u03b1) and human SDF\u20101\u03b1\u2010 nuclear factor \u03baB plasmid (phSDF\u20101\u03b1\u2010NF\u03baB), were constructed and loaded onto cationic microbubble carriers, and the plasmids were released into MI rabbits by ultrasound\u2010targeted microbubble destruction. The transfection efficiency of SDF\u20101\u03b1 and the degree of heart repair were further explored and compared. In the MI rabbit models, transfection with phSDF\u20101\u03b1\u2010NF\u03baB resulted in higher SDF\u20101\u03b1 expression in peri\u2010infarct area compared with transfection with phSDF\u20101\u03b1 or no transfection. Upregulation of SDF\u20101\u03b1 was shown beneficial to these MI rabbit models, as demonstrated with better recovery of cardiac function, greater perfusion of the myocardium, more neovascularization, smaller infarction size and thicker infarct wall 1\u2009month after treatment. Ultrasound\u2010targeted cationic microbubbles combined with the NF\u03baB binding motif could increase SDF\u20101\u03b1 gene transfection, which would play a protective role after MI.<\/jats:p>","DOI":"10.1002\/kjm2.12529","type":"journal-article","created":{"date-parts":[[2022,3,24]],"date-time":"2022-03-24T21:54:09Z","timestamp":1648158849000},"page":"594-604","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Ultrasound\u2010targeted cationic microbubbles combined with the <scp>NF\u03baB<\/scp> binding motif increase <scp>SDF<\/scp>\u20101\u03b1 gene transfection: A protective role in hearts after myocardial infarction"],"prefix":"10.1002","volume":"38","author":[{"given":"Cai\u2010Gui","family":"Yu","sequence":"first","affiliation":[{"name":"Department of Echocardiography Renmin Hospital of Wuhan University  Wuhan Hubei 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