{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T06:14:05Z","timestamp":1759990445670,"version":"3.40.5"},"reference-count":39,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2023,2,2]],"date-time":"2023-02-02T00:00:00Z","timestamp":1675296000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Kaohsiung J of Med Scie"],"published-print":{"date-parts":[[2023,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Insulin receptor substrate 1 and 2 (IRS1\/2) have been found involved in many cancers development and their inhibitors exert significant tumor\u2010suppressive effects. Here, we tried to explore the function of NT157, an IGF1R\u2010IRS1\/2 inhibitor, in ovarian cancer. We treated ovarian cancer cells with varying doses of NT157. The MTT assay was employed to evaluate cell proliferation and colony formation assay was used for detecting colony\u2010forming ability. TUNEL assay was adopted to test cell apoptosis. Cell invasion was checked by the Transwell assay. The expression of apoptosis\u2010related proteins, autophagy markers, IRS1\/2, and PI3K\/AKT\/mTOR pathway was compared by Western blot, immunofluorescence, or qRT\u2010PCR. As indicated by the data, NT157 abated the viability, proliferation, and induced autophagy of ovarian cancer cells. Overexpressing IRS1\/2 attenuated the tumor\u2010suppressive effect of NT157 and heightened the PI3K\/AKT\/mTOR pathway activation. Inhibition of the PI3K\/AKT\/mTOR pathway enhanced the tumor\u2010suppressive effect of NT157 and facilitated NT157\u2010mediated autophagy. However, the autophagy inhibitor 3\u2010MA partly reversed NT\u2010157\u2010mediated antitumor effects. In conclusion, this study disclosed that NT157 suppressed the malignant phenotypes of ovarian cancer cells by inducing autophagy and hampering the expression of IRS1\/2 and PI3K\/AKT\/mTOR pathway.<\/jats:p>","DOI":"10.1002\/kjm2.12652","type":"journal-article","created":{"date-parts":[[2023,2,2]],"date-time":"2023-02-02T15:08:18Z","timestamp":1675350498000},"page":"377-389","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Repressing <scp>IRS1<\/scp>\/2 by <scp>NT157<\/scp> inhibits the malignant behaviors of ovarian cancer through inactivating <scp>PI3K<\/scp>\/<scp>AKT<\/scp>\/<scp>mTOR<\/scp> pathway and inducing autophagy"],"prefix":"10.1002","volume":"39","author":[{"given":"Cai\u2010Xia","family":"Li","sequence":"first","affiliation":[{"name":"Department of Gynaecology and Obstetrics Shanghai Tenth People's Hospital Affiliated to Tongji University  Shanghai 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