{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T03:08:55Z","timestamp":1791083335097,"version":"4.1.0"},"reference-count":30,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2023,2,9]],"date-time":"2023-02-09T00:00:00Z","timestamp":1675900800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Kaohsiung J of Med Scie"],"published-print":{"date-parts":[[2023,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Atopic dermatitis (AD) is a common inflammatory skin disease. Matrine is the main component of the traditional Chinese medicine\n                    <jats:italic>Sophora flavescens<\/jats:italic>\n                    , and it poses good therapeutic effects on inflammatory diseases. This study aimed to explore the pharmacological effects of matrine on AD and its underlying mechanism. An AD mouse model and inflamed human epidermal keratinocyte cells (HaCaT) cells were established. Histopathological aspects were examined using hematoxylin and eosin staining, toluidine blue staining, and immunohistochemistry. The mRNA and protein expressions were assessed using quantitative real\u2010time polymerase chain reaction and Western blot, respectively. The secretions of cytokines and chemokines were examined by enzyme\u2010linked immunosorbent assay. Flow cytometry was carried out to analyze the proportions of T\u2010helper (Th) 1 and Th2 cells. Herein, our results displayed that matrine diminished AD symptoms and decreased heat shock protein 90 (Hsp90) expression. Matrine decreased the Th2 cytokine levels in the ear tissues and serum, and it also significantly repressed inflammatory cytokines (thymus activation regulated chemokine and interleukin\u20106) secretions by repressing the Hsp90\/NF\u2010\u03baB signaling axis in inflamed HaCaT cells. Furthermore, matrine inhibited Th2 differentiation of CD4\n                    <jats:sup>+<\/jats:sup>\n                    T cells when co\u2010cultured with inflamed HaCaT cells. Matrine can regulate the Th1\/Th2 inflammatory response by inhibiting the Hsp90\/NF\u2010\u03baB signaling axis to alleviate AD. Therefore, it may be a candidate for AD treatment.\n                  <\/jats:p>","DOI":"10.1002\/kjm2.12655","type":"journal-article","created":{"date-parts":[[2023,2,9]],"date-time":"2023-02-09T06:55:01Z","timestamp":1675925701000},"page":"501-510","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Matrine regulates Th1\/Th2 inflammatory responses by inhibiting the Hsp90\/\n                    <scp>NF\u2010\u03baB<\/scp>\n                    signaling axis to alleviate atopic dermatitis"],"prefix":"10.1002","volume":"39","author":[{"given":"Pan","family":"Huang","sequence":"first","affiliation":[{"name":"Department of Dermatology Second Affiliated Hospital of Hunan University of Traditional Chinese Medicine  Changsha Hunan Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fan","family":"Hu","sequence":"additional","affiliation":[{"name":"Department of anorectal Second Affiliated Hospital of Hunan University of Traditional Chinese Medicine  Changsha Hunan Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhi\u2010Bo","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Dermatology Second Affiliated Hospital of Hunan University of Traditional Chinese Medicine  Changsha Hunan Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi","family":"Pan","sequence":"additional","affiliation":[{"name":"Department of Dermatology Second Affiliated Hospital of Hunan University of Traditional Chinese Medicine  Changsha Hunan Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rong","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Dermatology Second Affiliated Hospital of Hunan University of Traditional Chinese Medicine  Changsha Hunan Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi\u2010Ning","family":"Yan","sequence":"additional","affiliation":[{"name":"Department of Dermatology Second Affiliated Hospital of Hunan University of Traditional Chinese Medicine  Changsha Hunan Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hai\u2010Zhen","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Dermatology Second Affiliated Hospital of Hunan University of Traditional Chinese Medicine  Changsha Hunan Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3156-5501","authenticated-orcid":false,"given":"Chang","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Dermatology Second Affiliated Hospital of Hunan University of Traditional Chinese Medicine  Changsha Hunan Province 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