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In this article, we constructed a rat developmental convulsion model and a primary hippocampal neuronal cell convulsion model, through which we studied hippocampal neuronal morphology and neuronal apoptosis using H&amp;E staining and TUNEL staining, respectively. Moreover, we measured TNF\u2010\u03b1, IL\u20106, and IL\u20101\u03b2 inflammatory factor levels using ELISA, MDA, and SOD kits. The expression of SIRT3 in hippocampal tissues was determined by qPCR and Western blotting. The expression of autophagy\u2010related proteins such as LC3, p62, and Beclin\u20101 was evaluated by Western blotting or immunohistochemistry. The role of SIRT3 and autophagic activity with Xyl\u2010B in convulsive seizure\u2010induced brain injury was investigated by knocking down SIRT3 expression levels. Our results showed that Xyl\u2010B plays a neuroprotective role in convulsive seizure\u2010induced brain injury by increasing SIRT3 expression and activating the autophagy pathway. The regulatory role of SIRT3 in the autophagy pathway with Xyl\u2010B treatment was explored by knocking down SIRT3 expression and inhibiting autophagy. Our results revealed that SIRT3 enhances the protective effect of Xyl\u2010B against postconvulsive brain injury by regulating AMPK\/mTOR signaling\u2010mediated autophagy.<\/jats:p>","DOI":"10.1002\/kjm2.12765","type":"journal-article","created":{"date-parts":[[2023,10,18]],"date-time":"2023-10-18T12:40:32Z","timestamp":1697632832000},"page":"74-85","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["<scp>SIRT3<\/scp> enhances the protective effect of <scp>Xyloketal B<\/scp> on seizure\u2010induced brain injury by regulating <scp>AMPK<\/scp>\/<scp>mTOR<\/scp> signaling\u2010mediated autophagy"],"prefix":"10.1002","volume":"40","author":[{"given":"Fen\u2010Fang","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Paediatrics The Second Affiliated Hospital of University of South China  Hengyang City Hunan Province 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